Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fifty Years and Counting: Searching for the "Silver Bullet" or the "Silver Shotgun" to Mitigate Preharvest Aflatoxin Contamination.

Toxins·2025
Same author

Population-specific pangenome unveils a third FAD2 gene and solves the peanut mid-oleic fatty acid mystery.

Nature communications·2025
Same author

Draft genome assemblies of 38 <i>Aspergillus parasiticus</i> isolates collected from South Georgia crop fields.

Microbiology resource announcements·2025
Same author

Development of a Quantitative PCR Method to Detect the Bacterial Gall Pathogen <i>Pseudomonas amygdali</i> pv<i>. loropetali</i> from Loropetalum Plant Materials.

Plant disease·2025
Same author

Host-Induced Gene Silencing of the <i>Aspergillus flavus O</i>-Methyl Transferase Gene Enhanced Maize Aflatoxin Resistance.

Toxins·2025
Same author

Field Evaluation of Experimental Maize Hybrids for Resistance to the Fall Armyworm (Lepidoptera: Noctuidae) in a Warm Temperate Climate.

Insects·2024

Related Experiment Video

Updated: Jun 19, 2025

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
10:01

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Published on: April 23, 2012

18.1K

Effects of Different Shelling Methods on Data Variability during Field Screening for Reduced Aflatoxin Contamination

Alison Adams1, Daniel Jeffers2, Shien Lu3

  • 1Department of Plant Pathology, University of Georgia, Griffin, GA 30223, USA.

Toxins
|July 26, 2024
PubMed
Summary

Shelling methods impact aflatoxin measurements in maize. Ear end removal (EER) and inoculation site-surrounding (ISS) methods reduce variability compared to whole ear (WE) shelling, aiding in identifying disease-resistant maize.

Keywords:
Aspergillus flavusaflatoxingermplasm screeningmaize breedingvariation

More Related Videos

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the &#945;-amylase Inhibitor from Lablab purpureus L.
09:21

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Published on: February 15, 2019

10.5K
High-throughput, Microscale Protocol for the Analysis of Processing Parameters and Nutritional Qualities in Maize Zea mays L.
05:55

High-throughput, Microscale Protocol for the Analysis of Processing Parameters and Nutritional Qualities in Maize Zea mays L.

Published on: June 16, 2018

6.9K

Related Experiment Videos

Last Updated: Jun 19, 2025

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
10:01

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Published on: April 23, 2012

18.1K
Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the &#945;-amylase Inhibitor from Lablab purpureus L.
09:21

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Published on: February 15, 2019

10.5K
High-throughput, Microscale Protocol for the Analysis of Processing Parameters and Nutritional Qualities in Maize Zea mays L.
05:55

High-throughput, Microscale Protocol for the Analysis of Processing Parameters and Nutritional Qualities in Maize Zea mays L.

Published on: June 16, 2018

6.9K

Area of Science:

  • Agricultural Science
  • Plant Pathology
  • Genetics and Breeding

Background:

  • Non-genetic variation complicates identifying maize germplasm with genetic resistance to Aspergillus flavus infection and aflatoxin contamination.
  • Aflatoxin measurements exhibit substantial variability within fields, even with identical maize germplasm, suggesting methodological influences beyond microenvironmental factors.

Purpose of the Study:

  • To evaluate the impact of three distinct maize shelling methods on aflatoxin measurement variability.
  • To determine if modified shelling techniques can improve the precision of phenotyping for Aspergillus flavus resistance in maize breeding.

Main Methods:

  • Maize ears from three inbred lines and two hybrids were inoculated with Aspergillus flavus.
  • Samples were subsequently shelled using three methods: whole ear (WE), ear end removal (EER), and inoculation site-surrounding (ISS).
  • Aflatoxin levels were quantified for each shelling method to calculate coefficients of variance (CVs).

Main Results:

  • Ear end removal (EER) and inoculation site-surrounding (ISS) shelling methods generally reduced CVs for aflatoxin measurements compared to whole ear (WE) shelling in both inbred and hybrid maize.
  • Exceptions included the susceptible B73 line, which showed increased CVs with EER and ISS, and the resistant Mp719 line, where EER marginally increased CVs.
  • Despite exceptions, EER and ISS demonstrate potential for more precise phenotyping than the standard WE method.

Conclusions:

  • Shelling methodology significantly influences the variability of aflatoxin measurements in maize.
  • Ear end removal (EER) and inoculation site-surrounding (ISS) offer improved precision for phenotyping maize germplasm for aflatoxin resistance compared to whole ear (WE) shelling.
  • These refined methods can aid in the accurate identification and selection of superior maize parental lines for breeding programs.