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Updated: Jun 19, 2025
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Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
Published on: June 30, 2016
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How c-di-GMP controls progression through the Streptomyces life cycle
Kelley A Gallagher1, Natalia Tschowri2, Richard G Brennan3
1Department of Microbiology, Cornell University, Ithaca, New York, USA.
Current Opinion in Microbiology
|July 26, 2024
Summary
Streptomyces bacteria use cyclic diguanylic acid (c-di-GMP) to control spore development. This molecule regulates key proteins like BldD, WhiG, and GlgX, guiding cell differentiation and spore maturation.
Area of Science:
- Microbiology
- Molecular Biology
- Biochemistry
Background:
- The bacterial genus Streptomyces exhibits a complex life cycle involving spore production.
- Cell differentiation in Streptomyces is tightly regulated by the second messenger 3', 5'-cyclic diguanylic acid (c-di-GMP).
Purpose of the Study:
- To elucidate the role of c-di-GMP in controlling the developmental life cycle of Streptomyces.
- To characterize proteins directly regulated by c-di-GMP in Streptomyces.
Main Methods:
- Functional and structural characterization of c-di-GMP-regulated proteins.
- Analysis of signaling pathways involving BldD, σWhiG, and GlgX.
Main Results:
- Identified BldD, σWhiG, and GlgX as key proteins directly controlled by c-di-GMP.
- Demonstrated c-di-GMP signaling through BldD and σWhiG controls aerial hyphae formation and sporulation.
- Showed c-di-GMP activates GlgX for glycogen degradation, supporting spore maturation.
Conclusions:
- c-di-GMP is a central regulator orchestrating critical developmental transitions in Streptomyces.
- The identified c-di-GMP effector proteins play distinct but coordinated roles throughout the Streptomyces life cycle.
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