Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

4.7K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surfactant-Assisted Construction of Covalent Organic Frameworks.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Indirect Effect Model with Surge Function for Describing Melatonin Circadian Rhythm: Quantitative Comparison and Application between Normal Sleepers and Patients with Delayed Sleep-Wake Phase Disorder.

Neuroendocrinology·2025
Same author

PARP Inhibitors Rechallenge in Patients With Recurrent Ovarian Cancer: A Multicentre Real-World Study in China.

BJOG : an international journal of obstetrics and gynaecology·2025
Same author

Correlation between visceral fat area and cardiac valve calcification in hemodialysis patients.

Frontiers in cardiovascular medicine·2025
Same author

Extrapolation of Midazolam Disposition in Neonates Using Physiological-Based Pharmacokinetic/Pharmacodynamic Modeling.

Clinical pharmacology in drug development·2025
Same author

Binding mechanism of metal ions (Ca<sup>2+</sup>, Cu<sup>2+</sup> and Mg<sup>2+</sup>) with tetrapeptide FFDR: A combined experimental and quantum chemistry approach.

Food chemistry·2025

Related Experiment Video

Updated: May 5, 2026

Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy
08:40

Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy

Published on: August 1, 2013

18.2K

"Boosting" tumor immunity in elderly mice by hyperactivating dendritic cells.

Jiaqi Liu1, Feng Shao2

  • 1Graduate Program in Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R. China; National Institute of Biological Sciences, Beijing, P.R. China.

Cell
|July 26, 2024
PubMed
Summary

Aging impairs tumor immunotherapy. Researchers found that hyperactivated dendritic cells, via the NLRP3 inflammasome, promote T-cell responses that eliminate tumors in aged mice, offering new therapeutic avenues.

More Related Videos

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

11.4K
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
04:29

Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

Published on: July 1, 2022

8.8K

Related Experiment Videos

Last Updated: May 5, 2026

Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy
08:40

Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy

Published on: August 1, 2013

18.2K
Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

11.4K
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
04:29

Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

Published on: July 1, 2022

8.8K

Area of Science:

  • Immunology
  • Cancer Research
  • Aging Biology

Background:

  • Immunosenescence, or immune system aging, significantly hinders effective tumor immunotherapy in elderly patients.
  • The cellular and molecular mechanisms underlying age-related immunotherapy failure require further elucidation.

Purpose of the Study:

  • To investigate how dendritic cell activation influences T-cell responses in the context of aging and cancer immunotherapy.
  • To explore the role of the NLRP3 inflammasome in mediating anti-tumor immunity in aged individuals.

Main Methods:

  • Utilized specific adjuvants to induce "hyperactivation" in dendritic cells.
  • Analyzed T-helper 1 (TH1)-skewed CD4+ T-cell responses.
  • Assessed tumor elimination in aged mouse models.

Main Results:

  • "Hyperactivated" dendritic cells promoted TH1-skewed CD4+ T-cell responses.
  • The NLRP3 inflammasome was critical for this adjuvant-induced immune response.
  • This mechanism led to the elimination of tumors in aged mice.

Conclusions:

  • Targeting dendritic cell activation through the NLRP3 inflammasome pathway presents a promising strategy for enhancing tumor immunotherapy in the elderly.
  • Understanding immunosenescence is key to developing effective cancer treatments for aging populations.