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Updated: Jul 11, 2026

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
Published on: January 4, 2017
Exclusion by the IncI plasmid R144 determined by measuring DNA transfer in Escherichia coli conjugations
Abstract:
Exclusion specified by the IncI plasmid R144 was determined by measuring the amount of donor DNA transferred to appropriate recipient cells. When recipient cells harboured an R144-derived Exc+ recombinant plasmid, the exclusion value determined in that way was comparable with the exclusion value determined by measuring the efficiency of transconjugant colony formation. When recipient cells harboured the plasmid R144drd-3, the exclusion value determined by measuring the amount of donor DNA transferred to recipient cells appeared more valid than the value determined by measuring transconjugant colony formation.
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