A Novel NIR Fluorescent Probe for Rapid Response to Hydrogen Sulfide

Area of Science:

• Chemical Biology
• Biomedical Engineering
• Analytical Chemistry

Background:

• Hydrogen sulfide (H2S) is a crucial small molecule bioregulator involved in numerous physiological processes.
• Aberrant H2S levels are linked to various pathological conditions, necessitating precise monitoring methods.

Purpose of the Study:

• To synthesize and characterize a novel near-infrared (NIR) fluorescent probe for sensitive and selective detection of H2S.
• To evaluate the probe's performance in terms of reaction kinetics, fluorescence response, detection limit, and selectivity.

Main Methods:

• Synthesis of a novel NIR fluorescent probe, DMT, based on Sulfur-substituted dicyanomethylene-4H-chromene (DCM).
• Investigation of the probe's reaction kinetics with H2S in weakly alkaline environments.
• Spectroscopic analysis to determine fluorescence enhancement, emission wavelength, detection limit, and Stokes shift.

Main Results:

• The DMT probe exhibited rapid reaction kinetics with H2S within 10 seconds.
• A significant fluorescence intensity enhancement (approximately 60-fold) was observed at 684 nm upon H2S interaction.
• The probe demonstrated a low detection limit (0.1623 µM), a large Stokes shift (94 nm), and excellent selectivity and anti-interference capabilities for H2S detection.

Conclusions:

• The developed DMT probe provides a sensitive, selective, and rapid method for visualizing H2S fluctuations.
• This novel probe holds promise for advancing the detection and application of H2S in biological and medical research.