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GRASLND regulates melanoma cell progression by targeting the miR-218-5p/STAM2 axis

Aiwei Ma1,2,3, Wenqi Shi1,2,3, Liyun Chen2,3,4

  • 1Department of Plastic Surgery and Burns Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, 515051, China.

Journal of Translational Medicine
|July 26, 2024
PubMed
Abstract

Insights

Long noncoding RNA GRASLND is upregulated in skin cutaneous melanoma (SKCM), promoting cell migration and invasion. Targeting the GRASLND/miR-218-5p/STAM2 axis may offer a new therapeutic strategy for SKCM.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are implicated in various biological processes and cancer.
  • The lncRNA GRASLND is recognized as an oncogene in multiple cancers.
  • The specific role of GRASLND in skin cutaneous melanoma (SKCM) remains underexplored.

Purpose of the Study:

  • To investigate the role and mechanism of lncRNA GRASLND in SKCM.
  • To explore the relationship between GRASLND, miR-218-5p, and STAM2 in SKCM.

Main Methods:

  • Assessed GRASLND expression in SKCM tissues and cell lines.
  • Utilized siRNA transfection to knockdown GRASLND.
  • Performed wound-healing and transwell assays to evaluate cell migration and invasion.
  • Investigated the interaction between GRASLND and miR-218-5p using binding assays and functional studies.

Main Results:

  • GRASLND expression is elevated in SKCM and correlates with poor prognosis and immunotherapy outcomes.
  • Knockdown of GRASLND significantly reduces SKCM cell migration and invasion.
  • GRASLND acts as a molecular sponge for miR-218-5p, leading to increased STAM2 expression.
  • GRASLND and miR-218-5p exhibit mutual inhibition.

Conclusions:

  • GRASLND promotes SKCM progression by regulating the miR-218-5p/STAM2 axis.
  • The lncRNA-miRNA-mRNA network involving GRASLND presents a potential therapeutic target for SKCM.
  • Further research into the GRASLND/miR-218-5p/STAM2 pathway could yield novel treatment strategies for melanoma.
Keywords:
BiomarkerImmunotherapyPrognosisSKCMlncRNA

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