Extracellular matrix hydrogels with fibroblast growth factor 2 containing exosomes for reconstructing skin...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Tissue Engineering

Background:

• Decellularized extracellular matrix (ECM) hydrogels offer biocompatibility for tissue repair but have limitations in biological activity and antimicrobial properties.
• Enhancing ECM hydrogels is crucial for improving their efficacy in clinical applications.

Purpose of the Study:

• To develop an improved ECM hydrogel with enhanced biological activity and antimicrobial properties for wound healing.
• To investigate the efficacy of fibroblast growth factor 2 (FGF 2) containing exosomes and copper ions in modifying ECM hydrogels.

Main Methods:

• Preparation of ECM hydrogel cross-linked with copper ions and loaded with FGF 2 containing exosomes (exoFGF2@ECM/Cu2+).
• In vitro assessment of cell proliferation, migration, antioxidant activity, and antibacterial effects.
• In vivo evaluation of wound healing in a rat model, including wound area measurements and histological analysis.

Main Results:

• The exoFGF2@ECM/Cu2+ hydrogel demonstrated excellent biocompatibility and antimicrobial activity in vitro.
• Significant promotion of cell proliferation, migration, and antioxidant effects was observed.
• In vivo studies showed accelerated wound closure (95.22% by Day 15) and enhanced angiogenesis and collagen deposition.

Conclusions:

• The developed exoFGF2@ECM/Cu2+ hydrogel effectively inhibits bacterial growth and promotes wound healing.
• This novel hydrogel shows significant potential for clinical applications in tissue repair and regeneration.