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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Correction: Renteria-Flores et al. Transforming Properties of E6/E7 Oncogenes from Beta-2 HPV80 in Primary Human Fibroblasts. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 5347.

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Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts
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The Fluorescent Cell Line SW620-GFP Is a Valuable Model to Monitor Magnetic Hyperthermia.

Saray Rosales1, Rodolfo Hernández-Gutiérrez2, Alma Oaxaca2

  • 1Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Marcelino García Barragan 1421, Guadalajara 44430, Jalisco, Mexico.

Bioengineering (Basel, Switzerland)
|July 27, 2024
PubMed
Summary

This study demonstrates magnetic hyperthermia effectively reduces tumor size in mice. The innovative use of fluorescent SW620-GFP cells allows for non-invasive monitoring of tumor response and cell viability.

Keywords:
fluorescencehyperthermiananoparticles

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Oncology

Background:

  • Magnetic hyperthermia offers a promising localized cancer treatment.
  • Iron oxide nanoparticles are key agents for magnetic hyperthermia.
  • Monitoring treatment efficacy non-invasively is crucial for in vivo studies.

Purpose of the Study:

  • To evaluate the efficacy of magnetic hyperthermia using folate-coated iron oxide nanoparticles in reducing SW620-GFP tumor xenografts.
  • To leverage the unique fluorescence of SW620-GFP cells for in vitro and in vivo monitoring of treatment response.
  • To characterize the magnetic nanoparticles for their suitability in hyperthermia applications.

Main Methods:

  • Preparation and characterization of folate-coated iron oxide nanoparticles with superparamagnetic properties.
  • In vitro assessment of cell viability using epifluorescence microscopy on SW620-GFP cells.
  • In vivo magnetic hyperthermia treatment of SW620-GFP tumors in mice, monitored non-invasively with iBox technology.

Main Results:

  • Characterization confirmed nanoparticles with superparamagnetic behavior, 12 ± 4 nm diameter, 2 nm coat, and high-power loss density.
  • Successful non-invasive monitoring of tumor size and shape in vivo using iBox technology.
  • Demonstrated significant tumor size reduction in mice after 3 weeks of magnetic hyperthermia treatment (3x/week).

Conclusions:

  • Folate-coated iron oxide nanoparticles are effective agents for magnetic hyperthermia.
  • The fluorescence of SW620-GFP cells provides a valuable tool for real-time monitoring of treatment efficacy.
  • Magnetic hyperthermia shows significant potential for non-invasive tumor size reduction in vivo.