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Related Concept Videos

Animal Mitochondrial Genetics02:59

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Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
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The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria.  In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
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Mitochondrial Protein Sorting01:39

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Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death.  Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
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  7. Medical Microbiology
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  11. Sequencing And Description Of The Mitochondrial Genome Of Orthopodomyia Fascipes (diptera: Culicidae)

Sequencing and Description of the Mitochondrial Genome of Orthopodomyia fascipes (Diptera: Culicidae)

Fábio Silva da Silva1,2, Bruna Laís Sena do Nascimento2, Ana Cecília Ribeiro Cruz1,2

  • 1Graduate Program in Parasitary Biology in the Amazon Region, Center of Biological and Health Sciences, State University of Pará, Belém 66095-663, Brazil.

Genes
|July 27, 2024

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Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila
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View abstract on PubMed

Summary
This summary is machine-generated.

This study presents the first mitochondrial genome for the mosquito Orthopodomyia fascipes, enhancing understanding of mosquito evolution. Phylogenetic analysis reveals new insights into the relationships within the Culicidae family.

Area of Science:

  • Genomics
  • Evolutionary Biology
  • Medical Entomology

Background:

  • The mosquito genus Orthopodomyia includes 36 species, primarily in tropical and temperate regions.
  • Limited knowledge of their bionomics and role in disease transmission hinders vector status assessment.
  • Scarcity of genetic data impedes understanding of their evolutionary relationships.

Purpose of the Study:

  • To describe the complete mitochondrial genome of Orthopodomyia fascipes, a Neotropical species.
  • To investigate the evolutionary relationships within the genus Orthopodomyia and the Culicidae family.
  • To contribute genetic information for improved taxonomy and evolutionary studies of mosquitoes.

Main Methods:

  • High Throughput Sequencing was employed to obtain the mitochondrial genome.
Keywords:
Culicidaemitogenomemolecular taxonomymosquitoes

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  • Phylogenetic analyses were conducted using Maximum Likelihood and Bayesian Inference.
  • The analysis utilized 13 protein-coding genes from the mitochondrial genome.

    • Main Results:

    • A complete mitochondrial genome sequence of 15,598 bp for Orthopodomyia fascipes was successfully obtained.
    • Phylogenetic analyses confirmed the monophyly of Culicidae and its subfamilies.
    • The study suggests a close relationship between the tribes Orthopodomyiini and Mansoniini, differing from some prior research.

    Conclusions:

    • The generated mitochondrial genome provides crucial data for Orthopodomyia taxonomy and evolutionary studies.
    • This research enhances the understanding of mosquito family Culicidae evolution.
    • The findings offer a foundation for future research on mosquito bionomics and disease transmission roles.
    phylogenetics