Sulforaphane Effects on Neuronal-like Cells and Peripheral Blood Mononuclear Cells Exposed to 2.45 GHz
Maria Paola Bertuccio1, Caterina Saija1, Giuseppe Acri1
1Department of Biomedical and Dental Sciences and Morpho-Functional Imaging, University of Messina, 98125 Messina, Italy.
International Journal of Molecular Sciences
|July 27, 2024
Summary
Low-dose sulforaphane protects neuronal and immune cells from electromagnetic radiation (EMR) damage. Higher doses, however, showed harmful effects, indicating a hormetic response to EMR exposure.
Area of Science:
- Neuroscience
- Cell Biology
- Biochemistry
Background:
- 2.45 GHz electromagnetic radiation (EMR) from common devices may cause oxidative stress.
- Neuronal-like cells and PBMCs are vulnerable to EMR-induced toxicity.
Purpose of the Study:
- To investigate sulforaphane's protective effects against 2.45 GHz EMR in neuronal-like cells and PBMCs.
- To evaluate EMR-induced changes in cell viability, mitochondrial function, and redox markers.
Main Methods:
- SH-SY5Y neuronal-like cells and PBMCs were exposed to 2.45 GHz EMR for 24 hours.
- Cells were treated with varying concentrations of sulforaphane (5-25 µg/mL).
- Assessed cell viability, mitochondrial activity, ROS production, NAD+/NADH ratio, and gene expression of redox and apoptosis markers.
Main Results:
- EMR exposure reduced cell viability and altered mitochondrial function and redox balance.
- A low concentration (5 µg/mL) of sulforaphane protected cells by reducing ROS and improving mitochondrial potential and NAD+/NADH ratio.
- Higher sulforaphane concentrations exhibited toxicity, demonstrating hormesis.
Conclusions:
- Neuronal-like cells are susceptible to EMR-induced mitochondrial dysfunction and oxidative stress.
- Low-dose sulforaphane shows potential in mitigating EMR-induced cellular damage.
- This study is the first to examine sulforaphane's effects on these cells under 2.45 GHz EMR exposure.
