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Bacterial Flora of the Large Intestine

Bacterial Flora of the Large Intestine

The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.

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An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota

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A Probiotic Amylase Blend Positively Impacts Gut Microbiota Modulation in a Randomized, Placebo-Controlled,

Mahmoud A Ghannoum^1,2, Mohammed Elshaer^1,2,3, Hilmi Al-Shakhshir¹

¹Department of Dermatology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA.

Life (Basel, Switzerland)

|July 27, 2024

Summary

A 6-week study found that a probiotic and amylase blend significantly increased beneficial yeast (Saccharomyces cerevisiae) and decreased potential pathogens in the gut microbiome. This blend also modulated gut bacteria, showing potential for microbiome realignment.

Keywords:

Candida bacteria fungi gut health microorganisms

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Published on: August 30, 2016

Area of Science:

Microbiology
Gastroenterology
Nutritional Science

Background:

Gut microbiota composition influences host health.
Probiotics and enzymes are explored for modulating the gut microbiome.
Limited research exists on the combined effects of probiotics and amylase.

Purpose of the Study:

To investigate the impact of a probiotic-amylase blend on gut microbiota abundance and diversity.
To assess changes in bacterial and fungal populations over a 6-week period.
To explore correlations between microbiota shifts and clinical features.

Main Methods:

A 6-week randomized clinical study involving subjects consuming a probiotic-amylase blend or a placebo.
Fecal sample analysis using 16S and ITS ribosomal RNA (rRNA) sequencing.
Assessment of bacterial and fungal organism abundance and diversity at baseline and post-intervention.

Main Results:

Significant 200-fold increase in Saccharomyces cerevisiae abundance and prevalence in the probiotic-amylase group (p ≤ 0.05).
Marked decrease in potential pathogens Bacillus thuringiensis (>150-fold) and Macrococcus caseolyticus (>175-fold).
Nine species (seven bacterial, two fungal) showed significant associations with 32 clinical features.

Conclusions:

Oral supplementation with a probiotic-amylase blend effectively increases beneficial yeast (Saccharomyces cerevisiae).
The blend modulates gut-dwelling commensal bacterial organisms and reduces potential pathogens.
Demonstrates proof of concept for using beneficial commensal organisms to realign gut microbiota.