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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: Jun 18, 2025

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

Label-Free Continuous Cell Sorting Using Optofluidic Chip.

Yingjie Zhang¹, Tao Zhang¹, Xinchun Zhang¹

¹Department of Mechanical Engineering, North China Electric Power University, Baoding 071003, China.

|July 27, 2024

Summary

This summary is machine-generated.

This study introduces a novel optical fiber tweezers system for non-invasive cell sorting. The developed optofluidic platform achieves high efficiency and cell viability without cell labeling, offering a cost-effective solution.

Keywords:

T-matrix cell sorting chip high viability label-free optofluidics scattering force

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Last Updated: Jun 18, 2025

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

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Published on: July 10, 2016

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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Area of Science:

Biomedical Engineering
Optics
Cell Biology

Background:

Efficient and non-invasive cell isolation is a critical challenge in biomedicine.
Optical fiber tweezers provide precise, non-invasive cell manipulation and integrate well with microfluidic systems.

Purpose of the Study:

To investigate cell manipulation using scattering force with optical fiber tweezers.
To develop and validate a novel optical fiber tweezers system for continuous cell sorting.

Main Methods:

Utilized flat-ended single-mode fiber for cell manipulation.
Derived a scattering force formula based on the T-matrix model.
Developed an optofluidic platform integrating optical tweezers with microfluidic chips featuring an expanded cross-channel design.

Main Results:

Successfully achieved continuous separation of yeast cells (8-10 µm) and polystyrene microspheres (15-20 µm).
Attained a sorting efficiency of up to 86%.
Maintained viability in approximately 90% of sorted yeast cells.

Conclusions:

The developed optical fiber tweezers system enables efficient, non-invasive, and continuous cell sorting.
This label-free system offers a cost-effective alternative to existing cell sorting technologies.
The optofluidic platform demonstrates significant potential for biomedical applications requiring precise cell manipulation.