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Liquid holding recovery in E. coli K12. I - Substances released during buffer holding
Revista Brasileira De Pesquisas Medicas E Biologicas
|June 1, 1979
Summary
Liquid holding recovery (LHR) in UV-irradiated E. coli cells increases viability. Researchers found LHR is dose-dependent and not always rec-gene dependent, suggesting DNA repair, membrane repair, or multiplication contribute to survival.
Area of Science:
- Microbiology
- Molecular Biology
- Cell Biology
Background:
- UV irradiation and nitrogen mustard induce DNA damage in E. coli.
- Liquid holding recovery (LHR) is a phenomenon where cell viability increases after incubation in a buffer post-treatment.
- The underlying mechanisms of LHR, particularly its dependence on rec-genes, require further investigation.
Purpose of the Study:
- To investigate cellular constituent release during LHR in UV-irradiated E. coli.
- To determine the dose-dependency of cellular release during LHR.
- To explore the role of rec-genes and caffeine in LHR and identify potential mechanisms for survival increase.
Main Methods:
- Incubation of UV-irradiated E. coli cells in a buffer.
- Monitoring of cellular constituent release during LHR.
- Treatment with nitrogen mustard and caffeine to assess LHR modulation.
- Analysis of dose-dependency of cellular release.
Main Results:
- Cellular constituent release during LHR was observed to be dose-dependent on UV irradiation.
- LHR was also induced by nitrogen mustard treatment.
- LHR was not inhibited by caffeine.
Conclusions:
- LHR is not exclusively dependent on rec-gene function.
- Survival increase during LHR may be attributed to DNA repair mechanisms.
- Reversible membrane damage and cellular multiplication are potential contributing factors to enhanced cell survival during LHR.