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Manganese-Loaded Liposomes: An In Vitro Study for Possible Diagnostic Application.

Maddalena Sguizzato1,2, Petra Martini3, Francesca Ferrara1

  • 1Department of Chemical, Pharmaceutical and Agricultural Sciences (DoCPAS), University of Ferrara, 44121 Ferrara, Italy.

Molecules (Basel, Switzerland)
|July 27, 2024
PubMed
Summary

Manganese (Mn)-based liposomal formulations show promise for diagnostic imaging like magnetic resonance imaging (MRI). These liposomes offer improved contrast and reduced toxicity compared to free manganese ions, suggesting suitability for diagnostic applications.

Keywords:
MEMRIcontrast agentsdiagnostic applicationliposomesmanganese

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Radiology

Background:

  • Free manganese ions (Mn2+) present toxicity concerns for diagnostic imaging.
  • Liposomal formulations offer a potential strategy to encapsulate and deliver manganese safely.
  • Developing safer contrast agents for magnetic resonance imaging (MRI) is crucial.

Purpose of the Study:

  • To investigate manganese-based liposomal formulations for diagnostic MRI applications.
  • To evaluate the safety and efficacy of encapsulated manganese compounds compared to free ions.
  • To assess the contrast enhancement properties of liposomal manganese formulations.

Main Methods:

  • Preparation and characterization of anionic liposomes loaded with manganese chloride (MC) and manganese hippurate (MH).
  • Evaluation of encapsulation efficiency using ICP-OES analysis.
  • Assessment of magnetic resonance imaging (MRI) contrast properties using phantoms and human cell safety tests.

Main Results:

  • High encapsulation efficiencies for both MC (99.7%) and MH (98.8%) within liposomes.
  • Liposomal manganese formulations (LP-MC, LP-MH) exhibited superior contrast-to-noise ratios compared to free Mn ions at equivalent concentrations.
  • The liposomal formulations demonstrated safety for use on human cells at the tested dose.

Conclusions:

  • Manganese-based liposomal formulations are effective carriers for diagnostic MRI.
  • These liposomes overcome the toxicity limitations of free manganese ions.
  • The enhanced contrast and safety profile suggest potential for preclinical diagnostic applications.