Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Plant Tissue Culture02:57

Plant Tissue Culture

37.7K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
37.7K
Primary and Secondary Growth in Roots and Shoots03:02

Primary and Secondary Growth in Roots and Shoots

57.0K
Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.
57.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surveillance for Unreported Vector-borne Viruses in the Republic of Korea, 2023-2025.

Public health weekly report·2026
Same author

Poly(glycerol)-modified liposome for co-delivery of pheophorbide a/ topotecan and combination cancer therapy in vivo.

Colloids and surfaces. B, Biointerfaces·2026
Same author

Pyramiding resistance to bacterial grain rot and bacterial leaf blight in rice using novel CAPS/dCAPS markers.

Plant physiology and biochemistry : PPB·2026
Same author

Long-term physical and mental health effects of unresolved disaster-related bereavement: evidence from an 8-year propensity score-matched cohort study.

European journal of psychotraumatology·2026
Same author

Reversibility and therapeutic feasibility of DNM1L-associated neurodevelopmental disorders.

Experimental & molecular medicine·2026
Same author

Structural brain MRI abnormalities in <i>SCN1A</i>-, <i>SCN2A</i>-, <i>SCN3A</i>-, and <i>SCN8A</i>-related epilepsies: a cohort study.

Frontiers in neurology·2026

Related Experiment Video

Updated: Jun 18, 2025

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System
07:00

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System

Published on: September 23, 2021

2.0K

High-Efficiency In Vitro Root Induction in Pear Microshoots (Pyrus spp.).

Jae-Young Song1, Jinjoo Bae1, Young-Yi Lee2

  • 1National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Suwon 16613, Republic of Korea.

Plants (Basel, Switzerland)
|July 27, 2024
PubMed
Summary

This study optimized in vitro pear propagation by using auxins for root induction, significantly improving rooting rates in various pear genotypes for commercial application.

Keywords:
IBANAAauxinplant growth regulatorroot induction

More Related Videos

Forced Flowering in Mandarin Trees under Phytotron Conditions
08:42

Forced Flowering in Mandarin Trees under Phytotron Conditions

Published on: March 6, 2019

9.0K
A Simple Protocol for Mapping the Plant Root System Architecture Traits
11:09

A Simple Protocol for Mapping the Plant Root System Architecture Traits

Published on: February 10, 2023

2.8K

Related Experiment Videos

Last Updated: Jun 18, 2025

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System
07:00

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System

Published on: September 23, 2021

2.0K
Forced Flowering in Mandarin Trees under Phytotron Conditions
08:42

Forced Flowering in Mandarin Trees under Phytotron Conditions

Published on: March 6, 2019

9.0K
A Simple Protocol for Mapping the Plant Root System Architecture Traits
11:09

A Simple Protocol for Mapping the Plant Root System Architecture Traits

Published on: February 10, 2023

2.8K

Area of Science:

  • Horticulture
  • Plant Biotechnology
  • Plant Physiology

Background:

  • Mass propagation of pear (Pyrus spp.) via tissue culture is efficient for shoot multiplication but hindered by low in vitro rooting rates.
  • Improving in vitro rooting is crucial for the commercialization and practical application of pear micropropagation.

Purpose of the Study:

  • To determine optimal conditions for inducing root formation in Pyrus microshoots.
  • To overcome the limitations of low rooting efficiency in pear in vitro culture.

Main Methods:

  • Microshoots were treated with a high concentration (2 mg L⁻¹) of auxins (IBA and NAA) at the onset of callus formation.
  • Treated microshoots were transferred to R1 medium (1/2 MS, 30 g L⁻¹ sucrose, no PGRs) for root development.

Main Results:

  • Successful rooting was achieved in European pear (P. communis), Asian pear (P. pyrifolia), and hybrid pear (P. pyrifolia × P. communis).
  • Rooting rates reached 66.7-100% for European, 60% for Asian, and 83.3% for hybrid pears within an average of 25 days.
  • Control groups showed significantly lower rooting rates (0-13.3%) after 60 days.

Conclusions:

  • The developed method effectively enhances in vitro root induction across diverse pear varieties.
  • This technique supports mass propagation and acclimatization of pear, with potential for global commercial use.