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Relationships within Bolbitis sinensis Species Complex Using RAD Sequencing.

Liyun Nie1,2,3, Yuhan Fang1, Zengqiang Xia1,4

  • 1Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

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The Bolbitis sinensis species complex is not composed of distinct species, according to phylogenetic and genetic analyses. Genetic differentiation varies among populations, influenced by geographic distance and gene flow.

Keywords:
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Area of Science:

  • Evolutionary Biology
  • Genetics
  • Botany

Background:

  • Species delimitation relies on accurate identification and phylogenetic relationships, which can be challenging using morphology, geography, and genomics.
  • Previous classifications of Bolbitis × multipinna and Bolbitis longiaurita, based on morphology, are phylogenetically linked to Bolbitis sinensis and do not form monophyletic groups.

Purpose of the Study:

  • To clarify the complex phylogenetic relationships within the Bolbitis sinensis species complex.
  • To investigate genetic diversity and differentiation across five populations of the Bolbitis sinensis complex.

Main Methods:

  • Restriction site-associated DNA (RAD) sequencing was employed on 65 individuals from five populations.
  • Phylogenetic trees, neighbor nets, and genetic structure analyses were integrated.

Main Results:

  • Phylogenetic and genetic analyses indicate that the Bolbitis sinensis complex does not represent separate species.
  • Genetic differentiation among the five populations ranged from low to moderate, influenced by geographical distance and gene flow.
  • Fst values confirmed that genetic differentiation increases with geographic distance.

Conclusions:

  • The study resolves the complex phylogenetic relationships within the Bolbitis sinensis species complex.
  • It elucidates genetic diversity and differentiation patterns, providing insights into evolutionary relationships and population genetics within the Bolbitis genus.