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Related Concept Videos

Equipments Used to Measure Body Temperature01:13

Equipments Used to Measure Body Temperature

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Body temperature can be assessed using various devices and measured in Celsius or Fahrenheit.
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...
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Temperature Measurement Sites01:14

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A thermometer measures body temperature. The common sites for measuring body temperature are the oral cavity, axillary region, temporal artery, and skin surface, such as the forehead, abdomen, and axilla. True core body temperature is assessed in the rectum, tympanic membrane, pulmonary artery, esophagus, and urinary bladder.
Oral: When assessing oral temperature, the thermometer tip should be placed under the tongue in the posterior sublingual pocket. It offers accurate readings and can be...
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Related Experiment Video

Updated: May 1, 2026

Frequency Mixing Magnetic Detection Scanner for Imaging Magnetic Particles in Planar Samples
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Isothermal Amplification Using Temperature-Controlled Frequency Mixing Magnetic Detection-Based Portable

Max P Jessing1,2, Abdalhalim Abuawad1,2, Timur Bikulov1,2

  • 1Institute of Biological Information Processing: Bioelectronics (IBI-3), Forschungszentrum Jülich, 52428 Jülich, Germany.

Sensors (Basel, Switzerland)
|July 27, 2024
PubMed
Summary
This summary is machine-generated.

This study shows how to control temperature for magnetic nucleic acid detection using frequency mixing magnetic detection (FMMD). This method enables reliable DNA amplification and detection in mobile, resource-limited settings.

Keywords:
frequency mixing magnetic detectionmagnetic nanoparticlespoint of care testingrecombinase polymerase amplificationthermal lumped parameter model

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Area of Science:

  • Biotechnology
  • Biosensing
  • Molecular Diagnostics

Background:

  • Sensitive magnetic nucleic acid (NA) detection using frequency mixing magnetic detection (FMMD) requires precise temperature control for amplified NA samples.
  • Recombinase polymerase amplification (RPA) is a crucial step for NA amplification but necessitates stable temperature conditions.

Purpose of the Study:

  • To investigate the feasibility of integrating RPA with a mobile FMMD setup for temperature-controlled NA amplification.
  • To develop and validate thermal models for accurate temperature control within the FMMD sensor unit under varying ambient conditions.

Main Methods:

  • Utilized the inherent heat generated by the low-frequency (LF) excitation signal of FMMD, controlled via pulse width modulation (PWM).
  • Developed steady-state and dynamic response models to predict thermal behavior at the sample position.
  • Validated RPA performance within the FMMD sensor unit against a standard temperature-controlled water bath.

Main Results:

  • Demonstrated successful temperature control for RPA within the FMMD sensor unit, achieving performance comparable to a water bath.
  • Developed accurate thermal models: linear extrapolation for narrow temperature ranges and a lumped parameter model (LPM) for broader conditions.
  • Confirmed the effectiveness of PWM for controlling temperature using the LF excitation signal's heat.

Conclusions:

  • The integrated FMMD system with PWM-based temperature control enables reliable RPA for nucleic acid amplification.
  • The developed thermal models accurately predict sample temperature, facilitating operation in diverse point-of-care (PoC) environments.
  • This approach holds promise for nucleic acid amplification and magnetic detection using FMMD in resource-limited settings.