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Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

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Related Experiment Video

Updated: May 2, 2026

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

Published on: May 15, 2014

Development of a Cell-Based Reporter Potency Assay for Live Virus Vaccines.

Dengyun Sun¹, Brian K Meyer¹, Dhanvanthri S Deevi²

¹Analytical Research & Development, Merck & Co., Inc., Rahway, NJ 07065, USA.

|July 27, 2024

Summary

A new cell-based reporter assay offers a faster, more reliable method for measuring the potency of live virus vaccines. This innovative assay using engineered cells provides accurate results, supporting vaccine development and quality control.

Keywords:

DoE qualification robustness vaccine potency assay

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Last Updated: May 2, 2026

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Area of Science:

Vaccinology
Virology
Biotechnology

Background:

Traditional vaccine potency assays like plaque or TCID50 are slow, labor-intensive, and variable.
Accurate potency measurement is crucial for life-saving vaccine development and release.

Purpose of the Study:

To develop and qualify a novel cell-based reporter potency assay for live virus vaccines.
To evaluate the reporter assay's performance for vaccines based on recombinant vesicular stomatitis virus (rVSV) and measles virus vectors.

Main Methods:

Utilized a Vero E6 cell line engineered to express NanuLuc® luciferase (JM-1 cells).
Measured viral infection intensity by quantifying NanuLuc® release into the supernatant post-infection.
Calculated relative potency using parallel line analysis (PLA) against a reference standard.

Main Results:

The reporter assay demonstrated good linearity, accuracy, and precision.
The assay showed robustness to parameter variations and correlated well with the traditional plaque assay for Vaccine 1.
The assay is suitable for supporting the manufacturing and release of multiple live virus vaccines.

Conclusions:

The developed reporter assay provides a more efficient and reliable alternative to traditional methods.
This cell-based reporter assay can be effectively used for quality control of live virus vaccines.
The JM-1 cell line facilitates the development of potent and safe vaccine products.