Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transient fluorescence in synchronously dividing Escherichia coli.

S P Layne, I J Bigio, A C Scott

    Proceedings of the National Academy of Sciences of the United States of America
    |November 1, 1985
    PubMed
    Summary

    Synchronous Escherichia coli (E. coli) cultures exhibit transient fluorescence during division, not distinct Raman spectra. This finding clarifies previous observations of bacterial spectral features, attributing them to fluorescence variations.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A prospective cohort study testing whether oral cavity mucosal tissue variability influences elastic scattering spectroscopy (ESS) measures.

    American journal of otolaryngology·2026
    Same author

    Elastic scattering spectroscopy for intraoperative oral cancer mucosal margin guidance: Initial results from a 104 patient cohort.

    American journal of otolaryngology·2025
    Same author

    Resolution by Gainesville Medical Society.

    Daniel's Texas medical journal·2023
    Same author

    Central Texas Medical Association.

    Texas medical journal (Austin, Tex.)·2023
    Same author

    Awake tracheal intubation during the COVID-19 pandemic - an aerosol-minimising approach.

    Anaesthesia reports·2020
    Same author

    Cancer treatment-related neuropathic pain: proof of concept study with menthol--a TRPM8 agonist.

    Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2015

    Area of Science:

    • Microbiology
    • Spectroscopy
    • Biophysics

    Background:

    • Previous laser-Raman spectroscopy studies on synchronous bacteria yielded complex spectral features.
    • The origin of these spectral features in previous studies remained unclear, potentially masking metabolic or cellular information.

    Purpose of the Study:

    • To investigate the Stokes laser-Raman spectra of metabolically synchronous Escherichia coli (E. coli).
    • To identify spectral lines attributable to the metabolic process or the cells themselves.
    • To understand the cause of previously observed spectral variations in synchronous bacterial cultures.

    Main Methods:

    • Utilized a spectrometer with an optical multichannel analyzer detector.
    • Recorded Stokes laser-Raman spectra of synchronous E. coli from 100 to 2100 cm-1.

    Related Experiment Videos

  • Performed over 400 recordings at cell concentrations of 10(7)-10(8) cells/ml.

    • Main Results:

    • No Raman lines directly attributable to the metabolic process or E. coli cells were detected.
    • Synchronous E. coli cultures displayed a reproducible, transient increase in fluorescence during a specific phase of the division cycle.
    • This fluorescence variation is hypothesized to be linked to changes in redox state or intracellular optical fields.

    Conclusions:

    • The observed spectral features in prior studies of synchronous bacteria are likely artifacts of time-dependent bacterial fluorescence, not intrinsic Raman signals.
    • Fluorescence variations, rather than metabolic Raman lines, are a significant spectral characteristic of synchronous E. coli during division.
    • Understanding these fluorescence dynamics is crucial for accurate interpretation of spectroscopic data from microbial systems.