Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of lanthanide extraction using dynamic interfacial tension measurement in the NaNO<sub>3</sub> aqueous solution/tributyl phosphate system.

Journal of colloid and interface science·2026
Same author

A desolvation-based molecular crowding mechanism revealed through α,β,γ,δ-tetrakis(1-methylpyridinium-4-yl)porphyrin <i>p</i>-toluenesulfonate-Zn complexation in alcohols.

Physical chemistry chemical physics : PCCP·2026
Same author

Solvent extraction mechanism through ibuprofen inclusion with β-cyclodextrin revealed by dynamic interfacial tension measurement.

Talanta·2026
Same author

Statistical quantification of SERS signals in microfluidic flow using AuNP-bound polystyrene microparticles.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2025
Same author

Intraparticle diffusion of proteins in diol group-functionalized porous silica particle revealed by fluorescence correlation spectroscopy.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2025
Same author

Detection scheme of arginine as aptamer target based on particle dissociation in coupled acoustic-gravitational field.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2025

Related Experiment Video

Updated: Jun 18, 2025

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
14:04

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

5.6K

DNA sensing based on aggregation of Janus particles using dynamic light scattering.

Akihisa Miyagawa1, Chisa Ito1, Yasuyuki Ueda2

  • 1Department of Chemistry, Faculty of Pure and Applied Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8577, Japan.

Analytica Chimica Acta
|July 27, 2024
PubMed
Summary

This study introduces a new DNA detection method using Janus particles (JPs) and dynamic light scattering (DLS). This approach enables sensitive detection of DNA sequences by monitoring JP aggregation, overcoming challenges with isotropic particle detection.

Keywords:
DNADynamic light scatteringJanus particleParticle assemblyParticle sizeSensing

More Related Videos

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
10:35

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Published on: November 9, 2017

12.1K
Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
09:16

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

14.3K

Related Experiment Videos

Last Updated: Jun 18, 2025

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
14:04

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

5.6K
DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
10:35

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Published on: November 9, 2017

12.1K
Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
09:16

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

14.3K

Area of Science:

  • Nanotechnology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Isotropic particle aggregation is difficult to control and quantify due to non-specific binding.
  • A novel detection scheme using Janus particles (JPs) with DNA tethered to one hemisphere was developed.

Purpose of the Study:

  • To develop a sensitive and quantitative method for DNA detection.
  • To overcome the limitations of detecting isotropic particle aggregation.

Main Methods:

  • Utilized Janus particles (JPs) with DNA on one hemisphere and hydrophobic properties on the other.
  • Employed dynamic light scattering (DLS) to monitor JP aggregation induced by DNA hybridization.
  • Applied a digital sensing method for quantitative analysis.

Main Results:

  • JP aggregation, monitored by DLS, indicated successful DNA detection.
  • Aggregation peaked at 2-3 particles, with further aggregation hindered by JP anisotropy.
  • Achieved sensitive DNA detection from pM to nM concentrations for simple and HIV-2 specific sequences.

Conclusions:

  • The anisotropic nature of JPs enables a novel aggregation-based detection method.
  • This scheme offers high sensitivity and broad applicability for detecting various molecules by modifying JPs.
  • Represents a significant advancement in particle-based sensing technologies.