Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

Biochemistry·2026
Same author

Neutralization of Reactive Oxygen Species with Cobinamide Augments Aortic Dissections and Rupture in BAPN-Administered Mice.

American journal of physiology. Heart and circulatory physiology·2026
Same author

A fatty acid amide activates myeloid cells and improves neurovascular outcomes in retinal degeneration.

Nature neuroscience·2026
Same author

Reactivity of Oxidized Porous Silicon with Calcium Ion in Aqueous Media: Stability, pH Behavior, and Host-Guest Strategies.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Cobinamide, a Vitamin B<sub>12</sub> Analog, Attenuates Benzo[a]pyrene and Pyrene Toxicity Through Selective Redox Modulation.

Toxics·2026
Same author

Disruptions in RNA Splicing: A Key Regulator of Cognitive Impairment in Perioperative Neurocognitive Disorders.

Neuroscience bulletin·2026
Same journal

Unlocking the capacity of Mn-based Prussian blue cathodes in capacitive deionization.

Nature communications·2026
Same journal

Scaling biodiversity-stability relationships from populations to meta-communities across trophic levels.

Nature communications·2026
Same journal

Thermodynamically programmed one-pot CRISPR platform for point-of-care SNP genotyping.

Nature communications·2026
Same journal

Engineering all-organic electrocatalysts with asymmetric dual-active sites for uncommon oxygen-evolving pathway.

Nature communications·2026
Same journal

Rapid GC content evolution in rice through GC-biased gene conversion and selection for translation efficiency.

Nature communications·2026
Same journal

Declines in organic matter persistence with increased soil carbon.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Jun 18, 2025

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies
12:38

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies

Published on: April 11, 2021

6.5K

Designed 2D protein crystals as dynamic molecular gatekeepers for a solid-state device.

Sanahan Vijayakumar1, Robert G Alberstein2, Zhiyin Zhang2

  • 1Materials Science and Engineering Program, University of California, San Diego, La Jolla, CA, 92093, USA.

Nature Communications
|July 27, 2024
PubMed
Summary
This summary is machine-generated.

Engineered protein crystals act as smart gates, selectively blocking or allowing molecules in response to chemical triggers like hydrogen cyanide (HCN). This innovation enables advanced sensor applications in non-aqueous environments.

More Related Videos

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature
07:42

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature

Published on: March 11, 2022

1.8K
Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
09:15

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering

Published on: August 14, 2018

10.5K

Related Experiment Videos

Last Updated: Jun 18, 2025

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies
12:38

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies

Published on: April 11, 2021

6.5K
On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature
07:42

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature

Published on: March 11, 2022

1.8K
Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
09:15

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering

Published on: August 14, 2018

10.5K

Area of Science:

  • Biomolecular Engineering
  • Materials Science
  • Chemical Sensing

Background:

  • Living cells' dynamic protein structures enable environmental responsiveness, motivating artificial protein assembly design.
  • Current designed protein assemblies lack integration into macroscale devices for practical applications.

Purpose of the Study:

  • To engineer a 2D crystalline protein assembly capable of selective molecular gating in response to a chemical trigger.
  • To demonstrate the integration of this protein assembly into a solid-state device for enhanced sensing capabilities.

Main Methods:

  • Engineered a 2D crystalline assembly of L-rhamnulose-1-phosphate aldolase (CEERhuA) using cobalt(II) coordination bonds.
  • Utilized a chemical trigger, hydrogen cyanide gas (HCN(g)), to induce conformational changes in the protein crystals.
  • Layered the 2D CEERhuA crystal onto a mesoporous silicon (pSi) photonic crystal optical sensor.

Main Results:

  • CEERhuA crystals transitioned from a closed state (<1 nm pores) to an ajar state (~4 nm pores) upon exposure to HCN(g).
  • The 2D CEERhuA layer selectively blocked interferents, preventing false positives on the pSi sensor.
  • The protein crystal layer opened upon exposure to low parts-per-million (ppm) levels of HCN(g), enabling analyte detection.

Conclusions:

  • Designed protein assemblies can function as dynamic, switchable components in solid-state devices.
  • This work demonstrates a novel approach for creating responsive materials for chemical sensing in non-aqueous environments.
  • The CEERhuA protein crystals offer a promising platform for advanced sensor technology.