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Hybrid Synthetic Promoters in Saccharomyces cerevisiae Built on Foreign Promoter Sequences
Xiaofan Feng1, Mario Andrea Marchisio2
1School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.
Abstract:
Traditionally, hybrid promoters are constructed, in Saccharomyces cerevisiae, by joining the core region and the upstream activating sequences from different native promoters. Here, we describe a new design that makes use of the core promoters from foreign organisms: viruses, humans, and the yeast Schizosaccharomyces pombe. With this approach, we realized a library of 59 new constitutive promoters that span over nine folds in gene expression.
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