Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

880
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
880

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Disrupted oxylipin biosynthesis mitigates pathogen infections and pest infestations in cotton (Gossypium hirsutum).

Journal of experimental botany·2024
Same author

Altered expression of SELF-PRUNING disrupts homeostasis and facilitates signal delivery to meristems.

Plant physiology·2023
Same author

Cotton phloem loads from the apoplast using a single member of its nine-member sucrose transporter gene family.

Journal of experimental botany·2021
Same author

Assessing Rates of Long-distance Carbon Transport in <i>Arabidopsis</i> by Collecting Phloem Exudations into EDTA Solutions after Photosynthetic Labeling with [<sup>14</sup>C]CO<sub>2</sub>.

Bio-protocol·2021
Same author

Assessing Long-distance Transport from Photosynthetic Source Leaves to Heterotrophic Sink Organs with [<sup>14</sup>C]CO<sub>2</sub>.

Bio-protocol·2021
Same author

Quantifying the Capacity of Phloem Loading in Leaf Disks with [<sup>14</sup>C]Sucrose.

Bio-protocol·2021

Related Experiment Video

Updated: Jun 18, 2025

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

9.5K

Cotton Meristem Transcriptomes: Constructing an RNA-Seq Pipeline to Explore Crop Architecture Regulation.

Róisín C McGarry1, Brian G Ayre2

  • 1Department of Biological Sciences and BioDiscovery Institute, University of North Texas, Denton, TX, USA. Roisin.McGarry@unt.edu.

Methods in Molecular Biology (Clifton, N.J.)
|July 27, 2024
PubMed
Summary
This summary is machine-generated.

Understanding plant meristems, the stem cells controlling growth, is key to improving crop yields. This study explores altering cotton meristem activity using virus-based technologies to enhance agricultural productivity.

Keywords:
CottonDevelopmentMeristemRNA-SeqStem cellsTranscriptomeVirusWGCNA

More Related Videos

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
07:18

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

7.4K
Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
08:33

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Published on: August 5, 2020

8.0K

Related Experiment Videos

Last Updated: Jun 18, 2025

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

9.5K
Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
07:18

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

7.4K
Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
08:33

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Published on: August 5, 2020

8.0K

Area of Science:

  • Plant Biology
  • Genetics
  • Agricultural Science

Background:

  • Plant meristems (stem cells) dictate growth patterns and organ size, influencing crop architecture and yield.
  • Cotton (Gossypium spp.) is a vital natural fiber crop, but its growth habits limit harvest yield and quality.
  • Understanding meristem regulation is crucial for improving cotton productivity and overcoming cultivation challenges.

Purpose of the Study:

  • To investigate the genetic networks regulating meristem activity in cotton.
  • To explore methods for altering cotton meristem dynamics to enhance yield and quality.
  • To establish a pipeline for analyzing transcriptomes from isolated cotton meristems.

Main Methods:

  • Utilized virus-based technologies to perturb signals controlling meristem fate and size in cotton.
  • Developed a pipeline for preparing and analyzing transcriptomes from isolated meristems.
  • Investigated changes in meristem dynamics following genetic perturbations.

Main Results:

  • Successfully altered cotton meristem dynamics using virus-based technologies.
  • Generated and analyzed transcriptomic data from isolated meristems.
  • Identified potential genetic networks influencing meristem activity and cotton growth.

Conclusions:

  • Virus-based technologies offer a viable approach to manipulate cotton meristem activity.
  • Altering meristem dynamics holds potential for improving cotton yield and quality.
  • Transcriptome analysis provides insights into the genetic regulation of cotton growth and development.