Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.0K
Histone Modification02:32

Histone Modification

3.4K
3.4K
Master Transcription Regulators02:23

Master Transcription Regulators

6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In Silico Identification and Functional Characterization of Genetic Variations across DLBCL Cell Lines.

Cells·2023
Same author

NetREx: Network-based Rice Expression Analysis Server for abiotic stress conditions.

Database : the journal of biological databases and curation·2022
Same author

Genome-wide characterization of copy number variations in diffuse large B-cell lymphoma with implications in targeted therapy.

Precision clinical medicine·2022
Same author

Sequence and Structure-Based Analyses of Human Ankyrin Repeats.

Molecules (Basel, Switzerland)·2022
Same author

DbStRiPs: Database of structural repeats in proteins.

Protein science : a publication of the Protein Society·2021
Same author

PRIGSA2: Improved version of protein repeat identification by graph spectral analysis.

Journal of biosciences·2020

Related Experiment Video

Updated: Jun 18, 2025

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer
10:36

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer

Published on: March 17, 2016

10.4K

Protocol for Analyzing Epigenetic Regulation Mechanisms in Breast Cancer.

Sri Lakshmi Bhavani Pagolu1, Nita Parekh2

  • 1Centre for Computational Natural Sciences and Bioinformatics, International Institute of Information Technology, Hyderabad, Telangana, India.

Methods in Molecular Biology (Clifton, N.J.)
|July 27, 2024
PubMed
Summary

This study explores DNA methylation

Keywords:
Breast cancerCorrelationDESeq2DNA methylationEpigeneticsGene expressionPromoterRnBeads

More Related Videos

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.2K
Methyl-binding DNA capture Sequencing for Patient Tissues
08:40

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

8.6K

Related Experiment Videos

Last Updated: Jun 18, 2025

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer
10:36

Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer

Published on: March 17, 2016

10.4K
Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.2K
Methyl-binding DNA capture Sequencing for Patient Tissues
08:40

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

8.6K

Area of Science:

  • Epigenetics
  • Cancer Biology
  • Bioinformatics

Background:

  • DNA methylation and gene expression are key epigenetic factors in cancer development.
  • Aberrant methylation patterns influence cancer transcriptome and have clinical implications.
  • Epigenetic changes affect tumor suppressor and oncogene function in tumorigenesis.

Purpose of the Study:

  • To present a unified workflow for investigating DNA methylation's role in gene expression regulation in breast cancer.
  • To identify differentially expressed genes linked to differential methylation in promoter or gene body regions.
  • To understand epigenetic mechanisms driving tumorigenesis through functional enrichment analysis.

Main Methods:

  • Utilizing Bioconductor packages within the R environment.
  • Analyzing genome-wide DNA methylation patterns.
  • Identifying differentially methylated and differentially expressed genes.
  • Performing functional enrichment analysis on identified gene sets.

Main Results:

  • Identification of differentially expressed genes associated with differential DNA methylation in breast cancer.
  • Linking specific methylation patterns to altered gene expression crucial for cancer.
  • Highlighting potential diagnostic and therapeutic targets through epigenetic analysis.

Conclusions:

  • The presented workflow effectively links DNA methylation patterns to gene expression changes in breast cancer.
  • Understanding these epigenetic alterations provides insights into cancer pathogenesis.
  • This approach aids in identifying potential biomarkers and therapeutic strategies for breast cancer.