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Proteomic analysis of brain tissue from ducks with meningitis caused by Riemerella anatipestifer infection

Guoliang Huang1, Shengmei Yang1, Ting Long1

  • 1Life Science and Engineering College, Northwest Minzu University, Lanzhou 730030, China.

Poultry Science
|July 28, 2024
PubMed

Insights

Riemerella anatipestifer causes meningitis in ducklings by crossing the blood-brain barrier (BBB). Proteomics identified proteins involved in transport and cell changes, suggesting bacterial entry via cellular and paracellular routes.

Area of Science:

  • Veterinary Microbiology
  • Proteomics
  • Neuroscience

Background:

  • Riemerella anatipestifer is a Gram-negative bacterium causing significant pathology in ducklings, including meningitis.
  • The precise mechanisms by which R. anatipestifer invades the central nervous system and causes meningitis remain unclear.
  • Understanding R. anatipestifer's blood-brain barrier (BBB) penetration is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the proteomic changes in duckling brain tissue following R. anatipestifer infection.
  • To identify key host proteins involved in R. anatipestifer's BBB penetration and meningitis development.
  • To elucidate the molecular pathways underlying R. anatipestifer-induced neurological disease.

Main Methods:

  • Establishment and characterization of a Riemerella anatipestifer-induced duck meningitis model.
  • 4D label-free quantitative proteomics analysis of infected duckling brain tissues.
  • Bioinformatics analysis of differentially expressed proteins, validated by RT-qPCR and Western-Blot.

Main Results:

  • Proteomic analysis revealed significant alterations in proteins related to intracellular, transport, and transmembrane transport activities.
  • Enrichment analysis indicated pathways involving reduced cell-cell adhesion and increased cell migration and apoptosis.
  • Identified proteins provide insights into potential mechanisms of bacterial entry into the brain.

Conclusions:

  • Riemerella anatipestifer likely penetrates the BBB through both transcellular and paracellular pathways.
  • The study provides the first proteomic insights into R. anatipestifer-induced meningitis in ducks.
  • Findings offer a foundation for future research into R. anatipestifer pathogenesis and BBB interactions.
Keywords:
Riemerella anatipestiferblood-brain barrierparacellular pathwayproteomicstranscellular pathway

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