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Published on: January 7, 2019
Fractionation of phenolic compounds from hickory by-products using solid phase extraction-sonication: Chemical
Changling Wu1, Bohui Ma1, David Julian McClements2
1Department of Food Science, Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang 311300, China; National Grain Industry (High-Quality Rice Storage in Temperate and Humid Region) Technology Innovation Center; Hangzhou 311300, China.
Abstract:
Hickory is an abundant source of phenolic compounds that exhibit a diverse range of bioactivities. In this study, phenolic compounds were extracted and purified from hickory green husk (HG), hickory nutshell (HN), and hickory seed coat (HS) using solid-phase extraction and ultrasonication (SPE-US). The effects of the SPE-US treatment on the structure and properties of the phenolic compounds were then investigated, including their composition, antioxidant activity, and antimicrobial activity. The dominant phenolic substances in the different extracts after SPE-US treatment were: ellagic acid and trans ferulic acid (HS); ellagic acid and sinapic acid (HN); and rutin (HG). The HS-SPE-US1 extract exhibited the highest total polyphenol content (416 ± 11 mg GAE/g DW), total flavonoid content (47.51 ± 0.68 mg RE/g DW), Fe3+ reduction ability (74.2 ± 1.0 mmol Fe2+/g DW), radical (DPPH and ABTS) scavenging ability, and antimicrobial activity against Staphylococcus aureus.
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