Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Receptor-mediated Endocytosis01:39

Receptor-mediated Endocytosis

104.3K
Overview
104.3K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.0K
Peroxisomes01:24

Peroxisomes

11.6K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
11.6K
Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

2.6K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
2.6K
Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

524
Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
524
Necrosis01:16

Necrosis

4.4K
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
4.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Smooth Muscle Cell Cytoglobin is a Negative Regulator of Atherosclerotic Fibrous Cap Development.

bioRxiv : the preprint server for biology·2026
Same author

Endothelial Cells at the Crossroad of Atherosclerosis and Metabolic Dysfunction-Associated Steatotic Liver Disease: A Unifying Target for Dual Treatment.

JACC. Basic to translational science·2026
Same author

Endothelial mitochondrial homeostasis in the development and progression of atherosclerosis.

Frontiers in cardiovascular medicine·2026
Same author

Eruptive zoophilic dermatophytosis and the expanding role of molecular diagnostics.

AME case reports·2026
Same author

Darier disease-A review highlighting new insights from the Darier Disease International Task Force.

Journal of the European Academy of Dermatology and Venereology : JEADV·2026
Same author

Chemical exposure and hepatic steatosis: How environmental factors impact liver health.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2026

Related Experiment Video

Updated: Jun 18, 2025

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

9.3K

Oxidized LDL regulates efferocytosis through the CD36-PKM2-mtROS pathway.

Jue Zhang, Jackie Chang, Vaya Chen

    Biorxiv : the Preprint Server for Biology
    |July 29, 2024
    PubMed
    Summary

    Macrophage efferocytosis, crucial for tissue health, is disrupted by oxidized LDL. This atherogenic lipoprotein overproduces mitochondrial ROS, impairing the clearance of apoptotic cells and driving atherosclerosis.

    More Related Videos

    LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring
    08:45

    LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

    Published on: November 17, 2018

    13.3K
    Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
    07:20

    Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

    Published on: January 31, 2025

    426

    Related Experiment Videos

    Last Updated: Jun 18, 2025

    Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
    07:29

    Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

    Published on: October 12, 2017

    9.3K
    LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring
    08:45

    LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

    Published on: November 17, 2018

    13.3K
    Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
    07:20

    Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

    Published on: January 31, 2025

    426

    Area of Science:

    • Immunology
    • Cell Biology
    • Cardiovascular Research

    Background:

    • Macrophage efferocytosis is vital for tissue homeostasis and preventing inflammation.
    • Dysfunctional efferocytosis contributes to atherosclerosis progression.
    • Oxidized LDL (oxLDL) is implicated in atherogenesis, but its precise effects on efferocytosis are unclear.

    Purpose of the Study:

    • To investigate the regulatory mechanisms of macrophage efferocytosis under atherogenic conditions.
    • To elucidate how oxLDL impacts efferocytosis and identify underlying molecular pathways.

    Main Methods:

    • Investigated macrophage efferocytosis in vitro.
    • Utilized oxidized LDL (oxLDL) and assessed mitochondrial reactive oxygen species (mtROS) production.
    • Examined the role of CD36, PKM2, and GRP75 in the efferocytosis pathway.
    • Analyzed the impact of oxLDL on apoptotic cell (AC) internalization.

    Main Results:

    • Macrophage mtROS normally facilitate efferocytosis via a positive feedback loop.
    • oxLDL disrupts efferocytosis by inducing excessive mtROS production.
    • oxLDL/CD36 signaling causes PKM2 translocation to mitochondria, enhancing mtROS generation.
    • This dysregulation impairs the internalization of ACs.

    Conclusions:

    • Macrophage efferocytosis is regulated by mitochondrial ROS.
    • Oxidized LDL disrupts efferocytosis through the CD36-PKM2-mtROS pathway.
    • This mechanism explains efferocytosis defects in atherosclerosis and suggests therapeutic targets.