Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bacterial RNA Polymerase

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Characterization and immunogenicity of nanoparticle vaccines displaying embecovirus spike proteins.

Biotechnology progress·2026

Same author

RBD-SD1 Nanoparticle Vaccines From DPP4-Using Merbecoviruses Elicit a Cross-Reactive Antibody Response but Limited Cross-Protective Immunity.

Biotechnology and bioengineering·2026

Same author

Hemagglutinin-displaying influenza nanovaccines: progress and promise.

Nanomedicine (London, England)·2025

Same author

Harnessing Plasmid Crosstalk in Transcription Factor Mediated Cell-Free Biosensors.

bioRxiv : the preprint server for biology·2025

Same author

Inverted H1 hemagglutinin nanoparticle vaccines protect mice against challenges with human H1N1 and bovine H5N1 influenza viruses.

NPJ vaccines·2025

Same author

Tethered Antigenic Suppression Shields the Hemagglutinin Head Domain and Refocuses the Antibody Response to the Stalk Domain.

Chemistry (Basel, Switzerland)·2025

Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026

Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026

Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026

Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026

Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026

Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 18, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

A modular cell-free protein biosensor platform using split T7 RNA polymerase.

Megan A McSweeney¹, Alexandra T Patterson¹, Kathryn Loeffler¹

¹School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, United States.

Biorxiv : the Preprint Server for Biology

|July 29, 2024

Summary

This summary is machine-generated.

A new biosensing platform, T7 RNA polymerase-Linked ImmunoSensing Assay (TLISA), enables rapid, equipment-free protein detection. This modular system is ideal for point-of-care diagnostics, offering flexibility and speed for biomarker analysis.

More Related Videos

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Published on: June 17, 2022

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

Related Experiment Videos

Last Updated: Jun 18, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Published on: June 17, 2022

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

Area of Science:

Biotechnology
Molecular Diagnostics
Biosensor Technology

Background:

Conventional protein detection methods are complex, costly, and time-consuming, limiting point-of-care applications.
Existing point-of-care assays often require lengthy development and lack flexibility for diverse targets.

Purpose of the Study:

To develop a modular, equipment-free biosensing platform for generalizable and rapid protein detection.
To demonstrate the flexibility and point-of-care suitability of the novel TLISA platform.

Main Methods:

Developed TLISA (T7 RNA polymerase-Linked ImmunoSensing Assay), a cell-free system using split T7 RNA polymerase fused to affinity domains.
Target antigen binding drives polymerase reassembly and reporter gene expression for detection.
Characterized platform modularity using 16 affinity domains against four antigens with minimal optimization.

Main Results:

Demonstrated TLISA's suitability for point-of-care use by detecting human biomarkers in serum and saliva within one hour.
Confirmed platform functionality after lyophilization, enhancing its potential for field applications.
Showcased minimal protocol optimization needed for diverse antigen detection, highlighting platform reconfigurability.

Conclusions:

TLISA offers a revolutionary approach to protein detection, enabling rapid, reconfigurable, and equipment-free diagnostics.
The platform's modularity and ease of use have significant potential for diverse point-of-care applications.
This technology could broadly impact infectious disease diagnostics, personalized medicine, and environmental monitoring.