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Related Concept Videos

Scale-Up Processes01:14

Scale-Up Processes

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The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
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Upstream Processing01:27

Upstream Processing

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Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
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Related Experiment Video

Updated: May 3, 2026

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
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Piloting a scale-up platform for high-quality human T-cells production.

Viknesvaran Selvarajan1, Denise Bei Lin Teo1, Chaw-Chiea Chang2

  • 1Food, Chemical and Biotechnology, Singapore Institute of Technology, Singapore, Singapore.

Frontiers in Cell and Developmental Biology
|July 29, 2024
PubMed
Summary

This study optimized adoptive T-cell therapy manufacturing. It identified key culture conditions for scalable, reproducible production of high-quality T-cells, crucial for advancing cancer treatment.

Keywords:
T-celladoptive cell therapybiaxial rotary bioreactorbioprocessingscale-upstirred-tank bioreactor

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Area of Science:

  • Immunotherapy
  • Cellular Therapy
  • Oncology

Background:

  • Adoptive cell therapy (ACT) is a promising cancer treatment, with CAR-T products driving market growth.
  • Standardizing ACT manufacturing is crucial for accessibility and reproducibility.
  • Understanding T-cell culture conditions is vital for effective expansion and differentiation.

Purpose of the Study:

  • To evaluate adoptive T-cell culture platforms for large-scale production.
  • To identify optimal conditions for high-quality cellular output with minimal cell death.
  • To address scalability and reproducibility challenges in ACT manufacturing.

Main Methods:

  • Assessed various adoptive T-cell culture platforms.
  • Examined bioreactor parameters, media, supplements, and stimulation.
  • Focused on achieving large-scale production (billions of cells) and high-quality output.

Main Results:

  • Identified critical factors influencing T-cell expansion and differentiation.
  • Demonstrated the potential for large-scale, reproducible T-cell manufacturing.
  • Achieved high-quality cellular output with minimal cell death across evaluated platforms.

Conclusions:

  • Optimized cell culture conditions are essential for robust ACT manufacturing.
  • Scalable and reproducible ACT production is achievable.
  • This research supports the advancement of ACT as a practical cancer therapeutic.