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Updated: Jun 18, 2025

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device
Published on: August 30, 2016
Epigenetic landscape of intestinal cell line HT29 cocultured with Lacticaseibacillus
Anunay Sinha1,2,3, Sanjeev Khosla1,3
1Centre for DNA Fingerprinting & Diagnostics, Hyderabad, 500039, India.
Abstract:
Aim: To investigate the changes in epigenetic landscape of HT29 cells upon coculture with the Lacticaseibacillus.Materials & methods: Histone and m6A mRNA modifications were examined by biochemical and NGS-based methods including western blotting, colorimetric assays, ChIP-Seq and direct mRNA sequencing. LC-MS was performed to identify Lacticaseibacillus secretome.Results: In cocultured HT29 cells global enrichment of H3K9ac and H3K4me3 and depletion of H3K9me3 mark was observed; mean genic positional signals showed depletion of H3K9ac and H3K4me3 at the TSS but enrichment in the upstream region; m6A methylation was altered in mRNAs corresponding to specific gene pathways; Lacticaseibacillus HU protein interacts with histone H3.Conclusion: Lacticaseibacillus can epigenetically alter specific genetic pathways in human intestinal cells.

