How to Prepare Neuroanatomical Image Data (an Update)

Image data from a single animal in neuroscientific experiments can be comprised of terabytes of information. Full studies can thus be challenging to analyze, store, view, and manage. What follows is an updated guide for preparing and sharing big neuroanatomical image data. © 2024 Wiley Periodicals LLC. Basic Protocol 1: Naming and organizing images and metadata Basic Protocol 2: Preparing and annotating images for presentations and figures Basic Protocol 3: Assessing the internet environment and optimizing images.