Human CKAP2L shows a cell cycle-dependent expression pattern and exhibits microtubule-stabilizing properties
Hyerim Kwon1, Jonathan Y Joh2, Kyung U Hong3
1School of Medicine, Sungkyunkwan University, Suwon, Korea.
FEBS Open Bio
|July 29, 2024
Summary
Cytoskeleton-associated protein 2-like (CKAP2L) plays a role in cell division. Elevated CKAP2L levels prolong mitosis by bundling microtubules, supporting its function in the human cell cycle.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Cytoskeleton-associated protein 2-like (CKAP2L) is a paralogue of CKAP2.
- CKAP2L's function in the human cell cycle requires further characterization.
Purpose of the Study:
- To characterize the expression pattern of CKAP2L.
- To determine the subcellular localization of CKAP2L.
- To investigate the microtubule-stabilizing properties of CKAP2L.
Main Methods:
- Analysis of CKAP2L transcript and protein levels across cell cycle phases and in human tissues.
- Detection of CKAP2L protein in various human cancer cell lines.
- Microscopy to determine subcellular localization during interphase and mitosis.
- Ectopic overexpression studies to assess the impact on microtubule dynamics and mitosis duration.
Main Results:
- CKAP2L expression is cell cycle-dependent, peaking in G2/M phase.
- CKAP2L is highly expressed in testis, intestine, and spleen, and detectable in all tested cancer cell lines.
- CKAP2L localizes to the mitotic spindle during mitosis and the nucleus during interphase.
- Overexpression of CKAP2L causes microtubule bundling and prolongs mitosis.
Conclusions:
- CKAP2L exhibits cell cycle-dependent expression and specific subcellular localization.
- CKAP2L possesses microtubule-stabilizing properties, influencing mitosis.
- These findings highlight the role of CKAP2L in regulating the human cell cycle.
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