Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and validation of an in-house one-step RT-qPCR method for the quantification of the BCR::ABL1 p210 transcripts.

Scientific reports·2026
Same author

Integrated experimental and mechanistic modelling for scenario-based assessment of flat-plate photobioreactors with mixed cultures of purple phototrophic bacteria.

Bioresource technology·2026
Same author

Interfacial removal of amoxicillin from water using a dendrimer-functionalized graphene-silica hybrid: mechanistic insights and validation in real samples.

Scientific reports·2026
Same author

WT1 as a Biomarker in Myelodysplastic Neoplasms: Clinical Correlations and Preliminary Data from an Iranian Cohort.

International journal of hematology-oncology and stem cell research·2026
Same author

Expression patterns of GPX4 and LINC00618 across disease phases in pediatric B-cell precursor acute lymphoblastic leukemia (BCP-ALL): an exploratory study.

BMC cancer·2026
Same author

Molecular genetic diagnosis of Bernard-Soulier syndrome in Iranian patients: reporting three novel mutations.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis·2026

Related Experiment Video

Updated: Jun 18, 2025

HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
11:29

HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells

Published on: July 20, 2016

11.0K

CDC27 gene expression patterns as a potential biomarker in Acute Leukemia.

Yasaman Pouriafar1, Shahrbano Rostami2, Nasrin Alizadghandforoush2

  • 1Department of Hematology and Blood Banking, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.

Molecular Biology Reports
|July 29, 2024
PubMed
Summary

Cell Division Cycle 27 (CDC27) is overexpressed in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). This suggests CDC27 may be a potential biomarker or therapeutic target for these blood cancers.

Keywords:
APC/CAcute lymphoblastic leukemiaAcute myeloid leukemiaCDC27Gene expression

More Related Videos

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

9.9K
Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances
07:35

Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances

Published on: October 11, 2018

7.4K

Related Experiment Videos

Last Updated: Jun 18, 2025

HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
11:29

HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells

Published on: July 20, 2016

11.0K
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

9.9K
Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances
07:35

Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances

Published on: October 11, 2018

7.4K

Area of Science:

  • Hematology
  • Oncology
  • Molecular Biology

Background:

  • Acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) treatments face challenges due to high relapse rates and drug resistance.
  • Cell cycle progression disruption is crucial in tumorigenesis.
  • The role of Cell Division Cycle 27 (CDC27) in the anaphase-promoting complex/cyclosome is known, but its significance in acute leukemia pathophysiology and as a biomarker is less understood.

Purpose of the Study:

  • To investigate the expression levels of CDC27 in patients with AML and ALL.
  • To evaluate the potential of CDC27 as a diagnostic and prognostic marker in acute leukemias.

Main Methods:

  • A case-control study involving 100 leukemia patients (50 AML, 50 ALL) and 50 healthy controls.
  • Quantitative real-time PCR (RQ-PCR) was used to analyze CDC27 expression.
  • Nonparametric Mann-Whitney U test was employed for statistical analysis.

Main Results:

  • AML and ALL patients exhibited significantly higher CDC27 expression compared to healthy individuals.
  • A weak correlation was observed between CDC27 expression and hematological parameters.
  • No significant correlation was found between CDC27 expression and sample type, demographics, clinical variables, or prognosis.

Conclusions:

  • CDC27 shows potential as an oncogene in acute leukemias.
  • CDC27 may serve as a valuable prognostic and diagnostic marker for AML and ALL.
  • Targeting CDC27 could be a promising therapeutic strategy for acute leukemias.