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Copper doped hydroxyapatite nanocomposite thin films: synthesis, physico-chemical and biological evaluation
Carmen Steluta Ciobanu1, Daniela Predoi2, Simona Liliana Iconaru1
1National Institute of Materials Physics, Atomistilor Street, No. 405A, P.O. Box MG 07, 077125, Magurele, Romania.
Summary
Copper-doped hydroxyapatite (CuHAp) thin films were successfully synthesized and characterized. These biocompatible CuHAp films exhibit excellent cell adhesion and proliferation, indicating potential for biomedical applications.
Area of Science:
- Materials Science
- Biomaterials Engineering
- Nanotechnology
Background:
- Hydroxyapatite (HAp) is a key biomaterial, but doping with copper (Cu) can enhance its properties.
- Developing advanced HAp-based materials is crucial for regenerative medicine and tissue engineering.
Purpose of the Study:
- To synthesize and characterize copper-doped hydroxyapatite (CuHAp) thin films.
- To evaluate the biocompatibility and cell interaction of CuHAp thin films.
Main Methods:
- Spin coating and sol-gel methods for CuHAp suspension preparation.
- Thermal treatment at 500 °C.
- Characterization using XRD, SEM, EDS, XPS, FTIR, Raman spectroscopy, DLS, zeta potential, ultrasound, and AFM.
- Cytotoxicity and cell proliferation assays with human gingival fibroblasts (HGF-1).
Main Results:
- XRD confirmed hexagonal hydroxyapatite structure.
- EDS and XPS verified copper ion incorporation.
- SEM and AFM revealed continuous, homogenous, and smooth thin film surfaces.
- DLS, zeta potential, and ultrasound confirmed suspension stability.
- CuHAp thin films demonstrated good biocompatibility, promoting HGF-1 cell adhesion and proliferation.
Conclusions:
- CuHAp thin films synthesized via spin coating are stable, homogenous, and biocompatible.
- The surface properties of CuHAp films effectively support human gingival fibroblast attachment and growth.
- These findings suggest CuHAp thin films are promising for biomedical applications, particularly in regenerative dentistry.
