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Differential expression of host oncogenes in human papillomavirus-associated nasopharyngeal and cervical epithelial cancers

  • 0Department of Biochemistry, Cell & Molecular Biology/West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana.
The Kaohsiung Journal of Medical Sciences +

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July 29, 2024

|

July 29, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

This study reveals that AKT mRNA is differentially expressed in cervical and nasopharyngeal cancers, unlike IQGAP1 and MMP16, irrespective of human papillomavirus (HPV) infection status.

Area Of Science

  • Oncology
  • Molecular Biology
  • Virology

Background

  • Human papillomavirus (HPV)-related cervical and nasopharyngeal cancers exhibit distinct molecular oncogenic pathways.
  • Differential expression of oncoproteins is a potential factor contributing to these disparities.

Purpose Of The Study

  • To investigate the expression patterns of host oncogenes (AKT, IQGAP1, MMP16) in HPV-associated cervical and nasopharyngeal cancers.
  • To compare these expression patterns between cancer tissues and non-cancerous tissues.

Main Methods

  • Tissue screening using Hematoxylin and Eosin staining.
  • HPV detection via PCR.
  • Protein expression analysis using immunohistochemistry.
  • mRNA profiling of oncogenes using RT-qPCR.

Main Results

  • AKT and IQGAP1 proteins were significantly upregulated in epithelial cancers compared to non-cancerous tissues.
  • IQGAP1 and MMP16 mRNA levels were higher in cancers, but AKT mRNA levels were not significantly different.
  • AKT mRNA levels were significantly elevated in cervical cancer (CC) compared to nasopharyngeal cancer (NPC).
  • No significant differences in protein levels of AKT, IQGAP1, and MMP16 were observed between CC and NPC.
  • Oncoprotein expression levels did not significantly differ between HPV-positive and HPV-negative cancer biopsies.

Conclusions

  • AKT mRNA exhibits differential expression in cervical and nasopharyngeal cancers, independent of HPV status.
  • IQGAP1 and MMP16 mRNA and protein expression patterns require further investigation in these cancer types.
  • The study highlights distinct molecular profiles that may influence cancer development and progression.

Keywords:

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