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Related Experiment Video

Updated: Jun 18, 2025

A Gusseted Thermogradient Table to Control Soil Temperatures for Evaluating Plant Growth and Monitoring Soil Processes

A Gusseted Thermogradient Table to Control Soil Temperatures for Evaluating Plant Growth and Monitoring Soil Processes

Published on: October 22, 2016

Using a thermal gradient table to study plant temperature signalling and response across a temperature spectrum.

Myrthe Praat^1,2, Zhang Jiang^1,2, Joe Earle^2,3

¹Plant Stress Resilience, Institute of Environmental Biology, Utrecht University, Padualaan 8, Utrecht, 3584CH, The Netherlands.

|July 29, 2024

Summary

Thermal gradient tables enable standardized plant studies across temperature ranges. This system allows researchers to investigate plant acclimation to heat (thermomorphogenesis) and cold, revealing dose-response effects on various traits.

Keywords:

Arabidopsis Cold acclimation Dose-response Lettuce Temperature Thermal gradient table Thermomorphogenesis Tomato

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Published on: January 30, 2020

Area of Science:

Plant Biology
Environmental Stress Physiology
Molecular Plant Science

Background:

Plants exhibit adaptive mechanisms like thermomorphogenesis (high temperature) and cold acclimation for optimal performance.
Understanding molecular pathways for acclimation to both heat and cold, and dose-response factors, remains challenging due to experimental limitations.
Simultaneous multi-condition growth is often impractical in standard labs.

Purpose of the Study:

To introduce and validate the use of thermal gradient tables for studying plant responses to a wide range of temperatures.
To demonstrate the system's capability in assessing plant acclimation and dose-response effects in a single experiment.
To provide technical guidance for implementing thermal gradient systems in plant research.

Main Methods:

Utilized commercially available thermal gradient tables to create a steep, adjustable temperature gradient for plant growth.
Assessed plant morphological, physiological, developmental, and molecular responses across the temperature gradient.
Quantified effects on seed germination, hypocotyl elongation, leaf development, rosette growth, gene expression, and physiological parameters.

Main Results:

Plants showed typical thermomorphogenesis and cold acclimation responses along the temperature gradient.
Demonstrated clear temperature dose-response effects on germination, growth, development, and molecular markers.
Confirmed the system's ability to induce and measure varied acclimation phenotypes.

Conclusions:

Thermal gradient tables provide a standardized, predictable, and efficient environment for studying plant temperature responses.
This technology facilitates research on temperature signaling, acclimation mechanisms, and dose-response relationships.
The system is readily implementable for advancing plant science research on environmental adaptation.