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In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this...
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A fiber-optic spectroscopic setup for isomerization quantum yield determination.

Anouk Volker1,2, Jorn D Steen1, Stefano Crespi1

  • 1Department of Chemistry - Ångström Laboratory, Uppsala University, Box 523, 751 20 Uppsala, Sweden.

Beilstein Journal of Organic Chemistry
|July 30, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a new spectroscopic setup for determining isomerization quantum yields. The method is reliable and accurate, matching literature values for azobenzene.

Keywords:
UV–vis spectroscopyisomerizationmolecular photoswitchesphotochemistryphoton flux

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Area of Science:

  • Photochemistry
  • Spectroscopy
  • Chemical Kinetics

Background:

  • Isomerization quantum yield is a critical parameter in photochemistry.
  • Accurate determination requires precise measurement of photon flux and spectral changes.
  • Existing methods can be complex or require specialized equipment.

Purpose of the Study:

  • To report a novel spectroscopic setup for isomerization quantum yield determination.
  • To validate the setup using a well-characterized photoswitch, azobenzene.
  • To assess the reliability and accuracy of the developed method.

Main Methods:

  • A spectroscopic setup combining fiber-coupled LEDs, a calibrated thermopile detector, and a UV-vis spectrometer was developed.
  • Photon flux was measured using the thermopile detector.
  • Isomerization quantum yields were calculated by numerically solving rate equations from UV-vis absorption spectra.

Main Results:

  • The setup successfully determined isomerization quantum yields for azobenzene.
  • Results were in excellent agreement with established literature values.
  • Error analysis indicated quantum yield values comparable to those obtained via actinometry.

Conclusions:

  • The reported spectroscopic setup provides a reliable and accurate method for determining isomerization quantum yields.
  • This approach offers a practical alternative for photochemical studies.
  • The validated setup demonstrates significant potential for future research in photoswitching systems.