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[Prospective use of immunofluorescence polarization in the study of enzymes modifying aminoglycosides]
Bollettino Dell'Istituto Sieroterapico Milanese
|January 1, 1985
Abstract:
A number of assays have been described for aminoglycoside-modifying enzymes; the aim of our investigation was to check the validity of polarized immunofluorescence assay for aminoglycoside enzymes. We have studied three enzymes encoded by R-factors from clinical isolates of Enterobacteriaceae conferring various patterns of aminoglycoside-resistance. The assay resulted valid: this is a simple, rapid and sensitive method.
Insights
Polarized immunofluorescence assay is a valid method for detecting aminoglycoside-modifying enzymes. This simple, rapid, and sensitive assay can be used for aminoglycoside resistance testing.
Area of Science:
- Microbiology
- Biochemistry
- Immunology