Multifunctional Bioactive Nanozyme Systems for Enhanced Diabetic Wound Healing

Area of Science:

• Biomaterials Science
• Nanotechnology
• Regenerative Medicine

Background:

• Diabetic wound healing is impaired by chronic inflammation and poor vascularization.
• Persistent oxidative stress and M1 macrophage dominance hinder the transition to proliferation.
• Current treatments often fail to address the complex pathophysiology of diabetic wounds.

Purpose of the Study:

• To engineer a nanozymatic therapeutic system for diabetic wound healing.
• To develop Janus nanoparticles (mSAM@aFGF) with catalase and superoxide dismutase-like activities.
• To create an injectable hydrogel (HAMA-mSAM@aFGF) for enhanced wound repair.

Main Methods:

• Synthesis of asymmetrically structured MnO₂-Au-mSiO₂@aFGF Janus nanoparticles (mSAM@aFGF).
• In vitro evaluation of nanozyme's antioxidant, anti-inflammatory, and pro-angiogenic properties.
• Development and characterization of a photocrosslinked hyaluronic acid hydrogel (HAMA) incorporating mSAM@aFGF.
• In vivo assessment of the hydrogel's efficacy in a diabetic wound model.

Main Results:

• mSAM@aFGF effectively scavenged reactive oxygen species (ROS) and generated oxygen.
• Nanozyme treatment enhanced cellular proliferation, migration, and angiogenesis in vitro.
• The HAMA-mSAM@aFGF hydrogel demonstrated injectability, adhesion, hemostasis, and anti-inflammatory effects.
• In vivo studies showed accelerated vascularization and M1 to M2 macrophage polarization, promoting wound healing.

Conclusions:

• The developed nanozymatic hydrogel system shows significant potential for managing diabetic wounds.
• This approach effectively addresses oxidative stress, inflammation, and vascularization deficits.
• The HAMA-mSAM@aFGF hydrogel offers a promising strategy to facilitate wound repair progression.