Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

16.9K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.9K
CRISPR01:59

CRISPR

50.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
50.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Silica's role in plant abiotic stress tolerance: A redox buffer hypothesis.

Plant physiology and biochemistry : PPB·2025
Same author

Cross-Activity Analysis of CRISPR/Cas9 Editing in Gene Families of <i>Solanum lycopersicum</i> Detected by Long-Read Sequencing.

Current issues in molecular biology·2025
Same author

Inferring single-cell and spatial microRNA activity from transcriptomics data.

Communications biology·2025
Same author

Targeting Tumor-Associated Sialic Acids Using Chimeric Switch Receptors Based on Siglec-9 Enhances the Antitumor Efficacy of Engineered T Cells.

Cancer immunology research·2024
Same author

Nicotinamide-Expanded Allogeneic Natural Killer Cells with CD38 Deletion, Expressing an Enhanced CD38 Chimeric Antigen Receptor, Target Multiple Myeloma Cells.

International journal of molecular sciences·2023
Same author

CRISPR-Cas9 engineering of the RAG2 locus via complete coding sequence replacement for therapeutic applications.

Nature communications·2023
Same journal

Correction to 'New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress'.

Nucleic acids research·2026
Same journal

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Nucleic acids research·2026
Same journal

Accessibility of telomeric overhangs to stabilizing small-molecule ligands.

Nucleic acids research·2026
Same journal

Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

Nucleic acids research·2026
Same journal

Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions and transcription start sites.

Nucleic acids research·2026
Same journal

Correction to 'The Gene Ontology knowledgebase in 2026'.

Nucleic acids research·2026
See all related articles

Related Experiment Video

Updated: Jun 18, 2025

Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting
07:01

Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting

Published on: May 2, 2025

106

Quantifying allele-specific CRISPR editing activity with CRISPECTOR2.0.

Guy Assa1, Nechama Kalter2, Michael Rosenberg2

  • 1Arazi School of Computer Science, Reichman University, Herzliya 4610101, Israel.

Nucleic Acids Research
|July 30, 2024
PubMed
Summary
This summary is machine-generated.

CRISPECTOR2.0 quantifies genome editing activity specific to each DNA allele. This tool addresses off-target effects by analyzing sequence variants for safer, personalized gene editing applications.

More Related Videos

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

21.6K
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

7.8K

Related Experiment Videos

Last Updated: Jun 18, 2025

Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting
07:01

Evaluation of Abnormal Growth-related Genes of Hematopoietic Stem and Progenitor Cells by Combining CRISPR/Cas9 Technology with Cell Counting

Published on: May 2, 2025

106
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

21.6K
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

7.8K

Area of Science:

  • Genetics and Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Off-target effects of CRISPR-Cas genome editing limit its safe application.
  • Genomic sequence variations influence on- and off-target editing profiles across alleles.
  • A tool for allele-specific genome editing quantification is currently unavailable.

Purpose of the Study:

  • Introduce CRISPECTOR2.0, an enhanced software for allele-specific genome editing quantification.
  • Enable precise, reference-free, and allele-aware measurement of on- and off-target activity.
  • Validate CRISPECTOR2.0 using diverse cell types and identify factors influencing allele-specific editing.

Main Methods:

  • CRISPECTOR2.0 utilizes de novo single nucleotide variant (SNV) detection.
  • Employs statistical-based allele-calling algorithms for precise quantification.
  • Analyzes data from human cell lines, primary human cells, and plants.

Main Results:

  • CRISPECTOR2.0 effectively quantifies allele-specific editing activity in samples with multiple alleles.
  • SNVs were identified to alter protospacer adjacent motif sequences, causing allele-specific editing.
  • Differential allelic editing was observed even with distal SNVs, suggesting epigenetic involvement.

Conclusions:

  • CRISPECTOR2.0 provides a reliable method for allele-specific genome editing quantification.
  • Understanding allele-specific editing is crucial for developing safe and personalized genome editing strategies.
  • Epigenetic factors may play a role in differential allelic editing outcomes.