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Transfer RNA Synthesis

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One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
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Related Experiment Video

Updated: Jun 18, 2025

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

RNA modifications identification based on chemical reactions.

Zhengjia Zhao¹, Weikai Yan¹, Xiaocheng Weng¹

¹Department of Clinical Laboratory, Center for Gene Diagnosis, and Program of Clinical Laboratory Medicine, Zhongnan Hospital of Wuhan University, China; College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan 430072, China.

Bioorganic & Medicinal Chemistry

|July 30, 2024

Summary

This summary is machine-generated.

This review details advancements in chemical labeling for RNA modification detection. It highlights methods for identifying ten RNA modifications and their sequencing applications, aiding future research.

Keywords:

Chemical labeling RNA modification Sequencing

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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

Area of Science:

Epigenetics
Molecular Biology
Chemical Biology

Background:

RNA modifications play crucial roles in cellular processes and gene regulation.
The discovery of numerous RNA modifications necessitates advanced detection techniques.
High-throughput sequencing methods are key for localizing and understanding modified RNAs.

Purpose of the Study:

To review recent progress in artificial chemical labeling and transformations for RNA modification identification.
To explore the chemical reactions underlying RNA modifications and their detection.
To compare the strengths and weaknesses of various chemical labeling-based sequencing methods.

Main Methods:

Comprehensive review of chemical labeling and transformation techniques for ten distinct RNA modifications.
Analysis of chemical reactions involved in RNA modification detection.
Comparison of antibody-based, enzymatic-based, and chemical-labeling-based high-throughput sequencing approaches.

Main Results:

Detailed overview of artificial chemical labeling strategies for various RNA modifications.
Discussion of the chemical principles and reaction mechanisms in RNA modification detection.
Introduction of methods for identifying multiple RNA modifications using chemical labeling.

Conclusions:

Chemical labeling offers powerful tools for RNA modification identification and sequencing.
Understanding the underlying chemical reactions is vital for developing accurate detection methods.
This review provides a resource for advancing RNA modification research and functional studies.