Co-localization and co-expression of Olfml3 with Iba1 in brain of mice
Himanshi Yadav1, Amrita Bakshi2, Anamika2
1Biochemistry and Molecular Biology Laboratory, Department of Zoology, Faculty of Science, University of Delhi, Delhi, India.
Abstract:
Olfml3 is a microglia-specific protein whose role in neuroinflammation is elusive. In silico analysis was conducted to characterize the Olfml3 protein, followed by molecular docking and MD simulation to check possible interaction with Iba1. Further, expression and co-localization analysis was performed in the LPS-induced neuroinflammatory mice brains. Results suggest that Olfml3 physically interacts with Iba1. Olfml3 and Iba1 expression increases during neuroinflammation in mice brains. Olfml3 was observed to co-localize with Iba1, and the number of Olfml3 and Iba1 dual-positive cells increased in the brain of the neuroinflammatory mice model. Thus, Olfml3 could potentially participate in microglia functions by interacting with Iba1.
Insights
Olfml3, a microglia protein, physically interacts with Iba1. Both proteins increase during neuroinflammation, suggesting Olfml3
Area of Science:
- Neuroscience
- Immunology
- Molecular Biology
Background:
- Microglia are key immune cells in the brain.
- The role of Olfml3 (Olfml3) in neuroinflammation is not well understood.
- Iba1 is a well-established marker for microglia activation.
Purpose of the Study:
- To investigate the role of Olfml3 in neuroinflammation.
- To determine if Olfml3 interacts with Iba1.
- To analyze the expression and localization of Olfml3 in a mouse model of neuroinflammation.
Main Methods:
- In silico analysis of Olfml3 protein structure.
- Molecular docking and molecular dynamics (MD) simulations.
- Expression and co-localization analysis in LPS-induced neuroinflammatory mouse brains.
Main Results:
- Olfml3 was predicted to physically interact with Iba1.
- Both Olfml3 and Iba1 expression levels were elevated in neuroinflammatory conditions.
- Olfml3 and Iba1 showed significant co-localization in microglia within the inflamed brain tissue.
Conclusions:
- Olfml3 physically interacts with Iba1.
- Olfml3 expression and co-localization with Iba1 increase during neuroinflammation.
- Olfml3 may play a role in microglia function through its interaction with Iba1.
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