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Microchip Liquid-Phase Ion Trap for Online Mass Spectrometry Analysis.
Guoxin Dai1, Lan Li2, Xianshuang Meng3
1School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China.
Analytical Chemistry
|July 31, 2024
Summary
Liquid-phase ion trapping (LPIT) on microfluidic chips enables precise ion manipulation. This technique enhances mass spectrometry sensitivity and separation for proteomics and chemical analysis.
Area of Science:
- Analytical Chemistry
- Microfluidics
- Mass Spectrometry
Background:
- Liquid-phase ion trapping (LPIT) offers potential for ion manipulation in liquid channels.
- LPIT coupled with mass spectrometry is promising for target enrichment and proteomics.
- Advancing LPIT requires flexible electric and fluid field designs.
Purpose of the Study:
- To construct and implement LPIT on microfluidic chips.
- To achieve nonlinear potential distribution for ion focusing.
- To enhance mass spectrometry detection sensitivity and separation capabilities.
Main Methods:
- Microfluidic chip fabrication utilizing conductive polymers.
- Generation of nonlinear potential distribution for ion manipulation.
- Integration with electrospray ionization and mass spectrometry.
- Theoretical analysis and numerical simulations for optimization.
Main Results:
- Demonstrated ion focusing based on effective charge and hydrodynamic radii.
- Achieved approximately 10-fold improvement in mass spectrometry detection sensitivity.
- Showcased excellent linearity (R² > 0.99) for reserpine quantification (1-100 ng/mL).
- Successfully demonstrated separation of an 11-amino acid mixture.
Conclusions:
- The developed LPIT microfluidic chip effectively enriches and separates ions.
- The technique significantly enhances mass spectrometry performance.
- This advancement holds promise for complex sample analysis in proteomics and beyond.
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