Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Sleep Apnea

Sleep Apnea

Sleep apnea is a condition where breathing stops intermittently during sleep, often leading to significant health issues. Each episode can last from 10 to 20 seconds or more and is frequently accompanied by a brief arousal from sleep. This disturbance, largely unnoticed by the individual, can lead to severe daytime fatigue. Commonly, individuals seek help after being informed by their partners about loud snoring and noticeable breathing pauses during sleep.
The condition is more prevalent among...

Cardiopulmonary Resuscitation II: ACLS Airway Management

Cardiopulmonary Resuscitation II: ACLS Airway Management

Airway management is a key skill in emergency and critical care settings, as maintaining a clear airway is essential for adequate oxygenation and ventilation.Head Tilt-Chin Lift TechniqueThe head tilt-chin lift maneuver is an essential technique primarily used in patients without suspected cervical spine injuries. To perform this maneuver, one hand is placed on the patient’s forehead, and gentle pressure is applied backward to tilt the head. The fingertips of the other hand are positioned under...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

High-Throughput Screening of Isomeric Reaction Products by Droplet Microfluidics Coupled to Cyclic Ion Mobility-Mass Spectrometry.

Analytical chemistry·2026

Same author

Barcoding for isobaric tagging of carboxylic acids and amines metabolites.

The Analyst·2026

Same author

Alkamines reveal a hidden layer of steroid and drug metabolism.

bioRxiv : the preprint server for biology·2026

Same author

Spatial Mapping of the Precancer-to-Cancer Transition in Breast and Prostate.

Cancer discovery·2026

Same author

SLC33A1 exports oxidized glutathione to maintain endoplasmic reticulum redox homeostasis.

Nature cell biology·2026

Same author

Metabolites from plasma-like medium fuel nitrogen metabolism and influence proliferation in <i>Leptospira interrogans</i>.

bioRxiv : the preprint server for biology·2026

Same journal

Heterojunction-Enhanced Interfacial Evanescent-Tunable Fiber Optic Probe for Amplification-free CRISPR/Cas12a-Based Rapid and Ultrasensitive Detection of MPXV.

Analytical chemistry·2026

Same journal

Tunable Charge Transfer in Europium Metal-Organic Frameworks for Ratiometric Sensing of a Sarin Simulant.

Analytical chemistry·2026

Same journal

A β-Cyclodextrin/Ag<sub>2</sub>O@MWCNT-Based Stochastic Platform for the Simultaneous Molecular Enantiorecognition and Enantioanalysis of Twelve Amino Acids in Biological Matrices.

Analytical chemistry·2026

Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026

Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026

Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 8, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

High-Throughput Metabolomics using 96-plex Isotope Tagging.

Michael R Armbruster¹, Scott F Grady¹, Kevin Cho²

¹Department of Chemistry and Biochemistry, Saint Louis University, 3501 Laclede Ave, St. Louis, Missouri 63103, United States.

Analytical Chemistry

|July 31, 2024

Summary

This summary is machine-generated.

New chemical tags accelerate liquid chromatography-mass spectrometry (LC-MS) metabolomics, enabling faster analysis and absolute quantitation. This method efficiently screens carboxylic acids in mammalian cells, overcoming previous workflow limitations.

More Related Videos

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: May 1, 2017

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: December 18, 2020

Related Experiment Videos

Last Updated: Jun 8, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: May 1, 2017

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: December 18, 2020

Area of Science:

Analytical Chemistry
Metabolomics
Biochemistry

Background:

Liquid chromatography-mass spectrometry (LC-MS) is a powerful metabolomics tool.
Current LC-MS workflows face challenges with long analysis times and variable quantitation.
Matrix effects and lengthy duty cycles hinder high-throughput metabolomic studies.

Purpose of the Study:

To develop novel chemical tags for improved LC-MS metabolomics.
To overcome the bottleneck of extended duty cycles in metabolomic workflows.
To enable absolute quantitation of metabolites, reducing matrix dependency.

Main Methods:

Synthesis and application of 96 unique chemical mass tags.
Utilizing LC-MS for high-throughput metabolic profiling.
Performing metabolic screening on nutrient-deprived mammalian cells.

Main Results:

Successfully developed and implemented 96 unique chemical mass tags.
Demonstrated alleviation of the metabolomic workflow bottleneck.
Achieved absolute quantitation of metabolites with reduced matrix effects.
Analyzed 288 samples in 2 hours with 24% relative standard deviation (RSD) for carboxylic acids.

Conclusions:

The novel chemical tags significantly enhance LC-MS metabolomics efficiency.
This approach allows for rapid and accurate absolute quantitation of metabolites.
The method is effective for metabolic screening in biological samples, such as mammalian cells.