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Updated: Jun 8, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
Published on: May 27, 2014
High-Throughput Metabolomics using 96-plex Isotope Tagging
Michael R Armbruster1, Scott F Grady1, Kevin Cho2
1Department of Chemistry and Biochemistry, Saint Louis University, 3501 Laclede Ave, St. Louis, Missouri 63103, United States.
None:
Liquid chromatography-mass spectrometry (LC-MS) based metabolomics suffers from extended duty cycles and matrix-dependent quantitation. Chemical tags with 96 unique masses are reported, which alleviate the metabolomic workflow bottleneck and allow for absolute quantitation. A metabolic screen for carboxylic acids was performed on mammalian cells deprived of various nutrients and showed 24% RSD and analysis of 288 samples in 2 h.
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