Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Free Ia E alpha chain expression in the E+ alpha : E- beta recombinant strain A.TFR5.

A B Begovich, P P Jones

|January 1, 1985

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Activation of RyR2 by class I kinase inhibitors.

British journal of pharmacology·2018

Same author

Sympathetic hyper-excitation in obesity and pulmonary hypertension: physiological relevance to the 'obesity paradox'.

International journal of obesity (2005)·2016

Same author

Association of IL2RA and IL2RB with rheumatoid arthritis: a replication study in a Dutch population.

Annals of the rheumatic diseases·2009

Same author

Factor V Leiden and thrombosis in patients with systemic lupus erythematosus: a meta-analysis.

Genes and immunity·2009

Same author

Detailed genetic characterization of the interleukin-23 receptor in psoriasis.

Genes and immunity·2008

Same author

Strategies and technical challenges in allele level Class II typing in 2578 bone marrow transplantation donor-recipient pairs.

Human immunology·2008

Low E alpha chain expression in A.TFR5 is due to the absence of E beta chains. This absence alters E alpha conformation and impairs its cell-surface transport.

Area of Science:

Immunogenetics
Molecular immunology
Biochemistry

Background:

The E alpha chain is a component of the E molecule, crucial for immune responses.
The recombinant strain A.TFR5 exhibits reduced E alpha chain expression.
A.TFR5 lacks E beta chains due to its genetic origin from the A.CA parent.

Purpose of the Study:

To investigate the molecular and biochemical basis for diminished E alpha chain expression in A.TFR5.
To elucidate the role of E beta in E alpha chain processing and cell-surface presentation.

Main Methods:

Restriction fragment length polymorphism (RFLP) analysis to map the recombination event.
Northern blot analysis to assess E alpha and E beta mRNA levels.
Two-dimensional gel electrophoresis of pulse-labeled proteins to analyze intracellular and cell-surface E alpha chain expression.

Related Experiment Videos

Main Results:

The recombination event in A.TFR5 is located upstream of the E alpha gene.
Normal E alpha mRNA levels but absent E beta mRNA were detected in A.TFR5.
A.TFR5 shows reduced intracellular E alpha levels and significantly decreased mature cell-surface E alpha protein compared to control strains.

Conclusions:

The absence of E beta chains in A.TFR5 leads to an altered conformation of the E alpha chain, reducing alloantibody recognition.
E beta is essential for the efficient processing and/or transport of the E alpha chain to the cell surface.
The defect in E alpha expression is post-transcriptional, affecting protein maturation and cell-surface localization.