Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

967
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
967
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

8.8K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
8.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Perioperative effects of caudal block on pediatric patients in laparoscopic upper urinary tract surgery: a randomized controlled trial.

BMC pediatrics·2019
Same author

Bone formation promoted by bone morphogenetic protein-2 plasmid-loaded porous silica nanoparticles with the involvement of autophagy.

Nanoscale·2019
Same author

Molecular identification guided process design for advanced treatment of electroless nickel plating effluent.

Water research·2019
Same author

Analyzing Complexity and Fractality of Glucose Dynamics in a Pregnant Woman with Type 2 Diabetes under Treatment.

International journal of biological sciences·2019
Same author

Time delay in seeking treatment for first-episode schizophrenia: a retrospective study.

Early intervention in psychiatry·2019
Same author

The S∴π hemibond and its competition with the S∴S hemibond in the simplest model system: infrared spectroscopy of the [benzene-(H<sub>2</sub>S) <sub></sub> ]<sup>+</sup> (<i>n</i> = 1-4) radical cation clusters.

Chemical science·2019

Related Experiment Video

Updated: Jun 18, 2025

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice
07:37

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

Published on: June 6, 2025

64

MicroRNA-19b exacerbates systemic sclerosis through promoting Th9 cells.

Yun-Ji Lim1, Sang-A Park1, Dandan Wang2

  • 1Mucosal Immunology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Drive, Bethesda, MD 20892, USA.

Cell Reports
|July 31, 2024
PubMed
Summary

MicroRNA-19b promotes T helper 9 cells, worsening systemic sclerosis (SSc). Inhibiting miR-19b ameliorates SSc in mice, suggesting a therapeutic target for this autoimmune disease.

Keywords:
CP: ImmunologyE2f8NF-κB p65TAK1TGF-β signalingTRAF6Th9miR-19bpatients with systemic sclerosis

More Related Videos

Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation
07:46

Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation

Published on: October 25, 2024

2.9K
Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice
08:09

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Published on: March 24, 2017

8.1K

Related Experiment Videos

Last Updated: Jun 18, 2025

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice
07:37

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

Published on: June 6, 2025

64
Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation
07:46

Author Spotlight: Achieving High-Purity In Vitro Differentiation of Th17 Cells Using Cytokine Concentration Modulation

Published on: October 25, 2024

2.9K
Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice
08:09

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Published on: March 24, 2017

8.1K

Area of Science:

  • Immunology
  • Molecular Biology
  • Autoimmune Diseases

Background:

  • Systemic sclerosis (SSc) is a complex autoimmune disorder marked by widespread fibrosis.
  • The precise immunological mechanisms driving SSc pathogenesis are not fully understood.

Purpose of the Study:

  • To investigate the role of microRNA-19b (miR-19b) in the development and progression of SSc.
  • To elucidate the molecular pathways through which miR-19b influences T helper 9 (Th9) cell activity in SSc.

Main Methods:

  • Utilized a bleomycin-induced mouse model of SSc.
  • Analyzed CD4+ T cells for miR-19b and IL-9 expression.
  • Investigated the molecular mechanisms involving TGF-β, IL-4, NLRC3, TRAF6, TAK1, NF-κB, and E2f8.
  • Correlated miR-19b and IL-9 levels with disease severity in SSc patients.

Main Results:

  • miR-19b and IL-9 levels are elevated in CD4+ T cells in experimental SSc.
  • Inhibition of miR-19b reduced Th9 cells and ameliorated SSc symptoms in mice.
  • A molecular pathway involving TGF-β, IL-4, NLRC3, TRAF6, TAK1, and NF-κB was identified, leading to miR-19b upregulation.
  • miR-19b directly upregulates IL-9 by suppressing E2f8.
  • Increased IL-9 and MIR-19B levels in SSc patients correlate with disease severity.

Conclusions:

  • miR-19b is a critical mediator in Th9 cell-driven SSc pathogenesis.
  • Targeting miR-19b presents a potential therapeutic strategy for managing SSc.