Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Viral Structure00:56

Viral Structure

62.0K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
62.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discovery, Structural Characterization, and Preclinical Evaluation of Monoclonal Antibodies against Xylazine Poisoning.

ACS pharmacology & translational science·2026
Same author

Development and characterization of mouse-adapted recombinant SARS-CoV-2 expressing reporter genes.

Microbiology spectrum·2026
Same author

Selective targeting of human TREX1 exonuclease by small molecule inhibitors is mediated by a conformational switch.

NAR molecular medicine·2026
Same author

Full-length structure of the anti-viral and pro-tumor DNA deaminase APOBEC3B.

bioRxiv : the preprint server for biology·2026
Same author

Sequence-directed covalent protein-RNA linkages in a single step using engineered HUH-tags.

Nucleic acids research·2026
Same author

Update and reuse: Structure-guided nanobody evolution against SARS-CoV-2 escape.

PLoS pathogens·2026

Related Experiment Video

Updated: Jun 18, 2025

Engineering Antiviral Agents via Surface Plasmon Resonance
13:00

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

2.3K

Structural basis for mouse receptor recognition by bat SARS2-like coronaviruses.

Wei Zhang1,2, Ke Shi3, Fu-Chun Hsueh1,2

  • 1Department of Pharmacology, University of Minnesota Medical School, Minneapolis, MN 55455.

Proceedings of the National Academy of Sciences of the United States of America
|July 31, 2024
PubMed
Summary

Rodent involvement in SARS-CoV-2 emergence is explored. Bat coronaviruses BANAL-236 and RaTG13 may have recombined in rodents, acquiring a furin motif and leading to SARS-CoV-2.

Keywords:
SARS-CoV-2X-ray crystallographyangiotensin-converting enzyme 2 (ACE2)receptor-binding domain (RBD)spike protein

More Related Videos

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.0K
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
08:40

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

58.9K

Related Experiment Videos

Last Updated: Jun 18, 2025

Engineering Antiviral Agents via Surface Plasmon Resonance
13:00

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

2.3K
Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.0K
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
08:40

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

58.9K

Area of Science:

  • Virology
  • Evolutionary Biology
  • Structural Biology

Background:

  • The precise animal origin of SARS-CoV-2 is still unknown.
  • SARS-CoV-2 spike protein shares similarities with bat coronaviruses BANAL-236 and RaTG13.
  • The presence of a furin motif in the SARS-CoV-2 spike is characteristic of rodent coronaviruses.

Purpose of the Study:

  • To investigate the potential role of rodents in the emergence of the SARS-CoV-2 spike protein.
  • To analyze the interaction between bat coronavirus spike proteins and mouse ACE2 receptors.

Main Methods:

  • Examined crystal structures of BANAL-236 and RaTG13 spike receptor-binding domains (RBDs) complexed with mouse ACE2.
  • Performed biochemical analyses to assess the binding affinity and receptor usage.

Main Results:

  • BANAL-236 and RaTG13 RBDs exhibit favorable residue alignments with mouse ACE2 binding hotspots.
  • Biochemical data confirm that BANAL-236 and RaTG13 spikes can utilize mouse ACE2 as an entry receptor.
  • Evidence suggests potential recombination events and furin motif acquisition in rodents.

Conclusions:

  • Bat coronaviruses like BANAL-236 and RaTG13 could have infected rodents.
  • Recombination in rodents may have led to the acquisition of a furin motif.
  • This process could have culminated in the emergence of SARS-CoV-2.