Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

50.4K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.4K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

12.5K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
12.5K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.1K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.1K
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

7.0K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.0K
Exon Recombination02:32

Exon Recombination

3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
3.6K
Base-pairing and DNA Repair02:27

Base-pairing and DNA Repair

64.7K
64.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polymerase theta repairs persistent G1-induced DNA breaks in S-phase during class switch recombination.

Nature communications·2025
Same author

Senataxin promotes recombination fidelity during antigen receptor gene diversification.

Science signaling·2025
Same author

JAK inhibition with tofacitinib rapidly increases contractile force in human skeletal muscle.

Life science alliance·2024
Same author

Increased AID results in mutations at the CRLF2 locus implicated in Latin American ALL health disparities.

Nature communications·2024
Same author

Tert-expressing cells contribute to salivary gland homeostasis and tissue regeneration after radiation therapy.

Genes & development·2024
Same author

Author Correction: Shifted PAMs generate DNA overhangs and enhance SpCas9 post-catalytic complex dissociation.

Nature structural & molecular biology·2024

Related Experiment Video

Updated: Jun 18, 2025

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.2K

ATM and 53BP1 regulate alternative end joining-mediated V(D)J recombination.

Jinglong Wang1, Cheyenne A Sadeghi1, Long V Le1

  • 1Division of Radiation and Cancer Biology, Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA.

Science Advances
|July 31, 2024
PubMed
Summary

Alternative end joining (A-EJ) in G0-G1 cells relies on a polymerase theta-independent Parp1-XRCC1/LigIII pathway, distinct from MMEJ used by cycling cells. This study identifies key DNA repair factors and mechanisms involved in this mutagenic process.

More Related Videos

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

9.1K
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

10.0K

Related Experiment Videos

Last Updated: Jun 18, 2025

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.2K
Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

9.1K
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

10.0K

Area of Science:

  • Molecular Biology
  • Genetics
  • DNA Repair Mechanisms

Background:

  • Alternative end joining (A-EJ) is a mutagenic DNA repair pathway.
  • A-EJ in G0-G1 cells differs from microhomology-mediated end joining (MMEJ) in cycling cells.
  • Understanding A-EJ is crucial for comprehending genome stability.

Purpose of the Study:

  • To systematically evaluate factors involved in G0-G1 A-EJ.
  • To elucidate the mechanism of A-EJ in V-J recombination and translocation.
  • To identify key DNA damage response (DDR) genes supporting A-EJ.

Main Methods:

  • Chemical and genetic approaches to assess A-EJ.
  • Mapping repair outcomes of RAG1/2-initiated double-strand breaks.
  • Investigating the roles of specific DDR genes (e.g., polymerase alpha, ATR, DNA2, Mre11).

Main Results:

  • A polymerase theta-independent Parp1-XRCC1/LigIII axis is central to A-EJ.
  • 53BP1 supports A-EJ within an ATM-activated DDR.
  • Compromising DDR factors like polymerase alpha, ATR, DNA2, and Mre11 alters resection, MMEJ, and translocation.

Conclusions:

  • The study defines key components and mechanisms of G0-G1 A-EJ.
  • It advances understanding of DNA repair within the 53BP1 regulatory domain.
  • Findings provide insights into the RAG1/2 postcleavage complex and its repair fates.