Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Brief Investigation: Investigating the role of phosphodiesterase Pde2 in coordinating the yeast Environmental Stress Response.

bioRxiv : the preprint server for biology·2026

Same author

Twenty-Five Years of the Environmental Stress Response and the Enduring Power of Yeast in Stress Biology.

Yeast (Chichester, England)·2026

Same author

Metagenomes and metagenome-assembled genomes from microbial communities in a biological nutrient removal plant operated at Hamptons Road Sanitation District (HRSD) with high and low dissolved oxygen conditions.

Microbiology resource announcements·2026

Same author

Using <i>Novosphingobium aromaticivorans</i> for Concurrent Production of Intracellular and Extracellular Products from Aromatics Extracted from Poplar Biomass.

ACS ES&T engineering·2026

Same author

The importance of regulated resource reallocation during dynamic environmental shifts in yeast.

The EMBO journal·2026

Same author

Genome sequences of nicotine-degrading <i>Pseudomonas</i> and <i>Stutzerimonas</i> strains isolated from nicotine-enriched activated sludge.

Microbiology resource announcements·2026

Search research articles

Related Experiment Video

Updated: Jun 18, 2025

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

Auto-MuRCiS: a streamlined software package for analysis of multiplex, randomized CRISPR interference sequencing

Kevin S Myers¹, Michael Place¹, Audrey P Gasch^1,2

¹Wisconsin Energy Institute and Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Microbiology Resource Announcements

|July 31, 2024

Summary

Multiplex, randomized CRISPR interference sequencing (MuRCiS) identifies multiple gene knockouts affecting microbial processes. Auto-MuRCiS is an updated Docker-based tool for rapid and user-friendly analysis of MuRCiS data.

Keywords:

CRISPRi computation docker pipeline python

More Related Videos

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

Related Experiment Videos

Last Updated: Jun 18, 2025

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

Area of Science:

Microbiology
Genomics
Bioinformatics

Background:

Multiplex, randomized CRISPR interference sequencing (MuRCiS) is a powerful technique for identifying genes involved in microbial processes.
Analyzing MuRCiS data can be complex and time-consuming, potentially limiting its widespread adoption.

Purpose of the Study:

To develop an updated, user-friendly analysis tool for MuRCiS data.
To streamline and accelerate the process of identifying multiple gene knockouts influencing microbial functions.

Main Methods:

Development of Auto-MuRCiS, a novel analysis tool.
Implementation of Docker for enhanced portability and ease of use.
Application of Auto-MuRCiS to analyze multiplex, randomized CRISPR interference sequencing data.

Main Results:

Auto-MuRCiS provides rapid analysis of MuRCiS data.
The tool enhances the user-friendliness of the MuRCiS data analysis workflow.
Successful identification of multiple gene knockouts impacting microbial processes.

Conclusions:

Auto-MuRCiS significantly improves the efficiency and accessibility of MuRCiS data analysis.
This tool facilitates deeper investigation into complex microbial genetic interactions.
The updated analysis pipeline supports broader research in microbial genomics and synthetic biology.